Lucas Schmidt

TL;DR: Live-cell observations of various modes of dynamic sarcomeric addition are reported, for the first time, and how these real-time images compare to static images from hypertrophic hearts reported in the literature are reported.

TL;DR: Results demonstrated that a single-cell can typically be patterned in 20-30 s, and that highly accurate and reproducible cellular arrays and systems can be achieved through coupling the microfluidics-based cell-delivery biochip with the laser guided system.

TL;DR: A microfluidic biochip to perform laser guidance on two cell types, chick embryonic forebrain neurons and spinal cord neurons, is expected to provide a new approach to high-throughput, label-free cell sorting with high sensitivity.

TL;DR: The laser cell-micropatterning system was used to place two neonatal CMs in contact on an aligned collagen gel to study the relationship between GJ maturation and BM development and showed that BM-laminin was deposited earlier than the formation of GJs in the intercellular space and that newly expressed connexin 43 clusters were preferentially assembled near the deposited BM structures.

TL;DR: This study reveals that 90% of the patterned cells maintained end-to-end connection 30 min after patterning, and mechanical junctions could be reinstalled between laser connected cells after overnight incubation, demonstrating that multiple beam laser patterning is an outstanding tool for in vitro studying contact-mediated cell-cell interactions among irregularly shaped cells.