Laser guidance-based cell detection in a microfluidic biochip
Wan Qin,Lucas Schmidt,Xiaoqi Yang,Lina Wei,Lina Wei,Ting Huang,Ting Huang,Julie X. Yuan,Xiang Peng,Xiaocong Yuan,Bruce Z. Gao +10 more
Reads0
Chats0
TLDR
A microfluidic biochip to perform laser guidance on two cell types, chick embryonic forebrain neurons and spinal cord neurons, is expected to provide a new approach to high-throughput, label-free cell sorting with high sensitivity.Abstract:
We developed a microfluidic biochip to perform laser guidance on two cell types, chick embryonic forebrain neurons and spinal cord neurons. Observation of neurons under a high-magnification microscope, which we obtained from these two cell types, showed no difference in morphology. However, when flowing in the microfluidic channel and simultaneously being laser guided, the two cell types gained quite different guidance speeds under the same experimental conditions. The results demonstrate that different cell types with the same morphology (e.g., size, shape, etc.) can be effectively distinguished from each other by measuring the difference in guidance speeds (the maximum flow speed minus the initial flow speed). This technique is expected to provide a new approach to high-throughput, label-free cell sorting with high sensitivity.read more
Citations
More filters
Journal ArticleDOI
Cerebral capillary velocimetry based on temporal OCT speckle contrast.
TL;DR: The proposed OCT capillary velocimetry method is applied to in vivo measurements of mouse brain, demonstrating that the proposed approach provides additional useful information in the quantitative assessment of capillary hemodynamics, complementary to that of OCT angiography.
Journal ArticleDOI
Hydrogel based 3D carriers in the application of stem cell therapy by direct injection
TL;DR: Compared with systematic administration such as peripheral intravenous infusion, stem cell therapy by direct injection is theoretically more effective, but some technical barriers such as low stem cell retention rate and low engraftment rate still need to be overcome before its application in humans.
References
More filters
Journal ArticleDOI
MitoTracker labeling in primary neuronal and astrocytic cultures: influence of mitochondrial membrane potential and oxidants.
Jennifer F. Buckman,Hélène Hernández,Geraldine J. Kress,Tatyana V. Votyakova,Sumon Pal,Ian J. Reynolds +5 more
TL;DR: Although MitoTrackers may have some value in identifying the location of mitochondria within cultured neurons and astrocytes, their sensitivity to Delta Psi(m) and oxidation negates their use as markers of mitochondrial dynamics in healthy cultures.
Book ChapterDOI
Flow cytometry and cell sorting.
TL;DR: Advances in computing and optics have led to a new generation of flow cytometers capable of processing cells at orders of magnitudes faster than their predecessors, and with staggering degrees of complexity, making the cytometer a powerful discovery tool in biotechnology.
Journal ArticleDOI
Microfluidics and photonics for Bio-System-on-a-Chip: a review of advancements in technology towards a microfluidic flow cytometry chip.
TL;DR: Some of the more recent developments in flow cytometry are reviewed to familiarize a reader with the current state of the technologies and also highlights the requirements of the device and how researchers are working to meet these needs.
Journal ArticleDOI
High-Throughput and High-Resolution Flow Cytometry in Molded Microfluidic Devices
Claire Simonnet,Alex Groisman +1 more
TL;DR: The design, fabrication, and operation of two types of flow cytometers based on microfluidic devices made of a single cast of poly(dimethylsiloxane) are described, which could be used for analysis of morphology and fluorescence distribution in cells in continuous flow.
Journal ArticleDOI
Recent advances in miniaturized microfluidic flow cytometry for clinical use
Taek Dong Chung,Hee Chan Kim +1 more
TL;DR: An overview of recent research achievements in miniaturized flow cytometry focuses on chip‐based microfluidic flow cytometers, classified by cell transport method, detection technology, and biomedical application.