M
M. Szardenings
Researcher at École Polytechnique Fédérale de Lausanne
Publications - 3
Citations - 227
M. Szardenings is an academic researcher from École Polytechnique Fédérale de Lausanne. The author has contributed to research in topics: Trichoderma reesei & Glycosidic bond. The author has an hindex of 3, co-authored 3 publications receiving 218 citations.
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The active site of cellobiohydrolase Cel6A from Trichoderma reesei: the roles of aspartic acids D221 and D175.
Anu Koivula,Laura Ruohonen,Gerd Wohlfahrt,Tapani Reinikainen,Tuula T. Teeri,K. Piens,M. Claeyssens,M. Weber,A. Vasella,D. Becker,M.L. Sinnott,J.Y. Zou,Gerard J. Kleywegt,M. Szardenings,Jerry Ståhlberg,T.A. Jones +15 more
TL;DR: Site-directed mutagenesis, X-ray crystallography, and enzyme kinetic studies have been used to confirm the role of residue D221 as the catalytic acid in Cel6A, and suggest that the single-displacement mechanism of Cel 6A may not directly involve a catalytic base.
Journal ArticleDOI
The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169
Anu Koivula,Tapani Reinikainen,Laura Ruohonen,Anne Valkeajärvi,Marc Claeyssens,Olle Teleman,Gerard J. Kleywegt,M. Szardenings,Juha Rouvinen,T.A. Jones,Tuula T. Teeri +10 more
TL;DR: Data suggest that Y169, on interacting with a glucose ring entering the second subsite in a narrow tunnel, helps to distort the glucose ring into a more reactive conformation, and catalytic constants towards cellotriose and cellotetraose are four times lower for the mutant.