M
Makoto Miyagishi
Researcher at National Institute of Advanced Industrial Science and Technology
Publications - 170
Citations - 19268
Makoto Miyagishi is an academic researcher from National Institute of Advanced Industrial Science and Technology. The author has contributed to research in topics: RNA interference & Small interfering RNA. The author has an hindex of 52, co-authored 161 publications receiving 18180 citations. Previous affiliations of Makoto Miyagishi include University of Tsukuba & Tohoku University.
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Journal ArticleDOI
Know-how of RNA interference and its applications in research and therapy
TL;DR: By virtue of their specific gene silencing activity and owing to the recent discoveries on their plasmid and virus driven expression, small dsRNAs are being widely adopted in research and therapeutics and are rapidly replacing the conventional gene knock-out technologies.
Journal Article
Escape from the interferon response associated with RNA interference using vectors that encode long modified hairpin-RNA
Hideo Akashi,Makoto Miyagishi,Takanori Yokota,Tsunamasa Watanabe,Taro Hino,Kazutaka Nishina,Michinori Kohara,Kazunari Taira +7 more
TL;DR: It is shown that modified hairpin-RNAs of more than 100 bp, with multiple specific point-mutations within the sense strand and transcribed from the U6 or tRNA(Val) promoters, can cause RNAi without inducing the IFN pathway genes.
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Identification of Metastasis-related Genes in a Mouse Model Using a Library of Randomized Ribozymes
Eigo Suyama,Eigo Suyama,Renu Wadhwa,Kamaljit Kaur,Makoto Miyagishi,Makoto Miyagishi,Sunil C. Kaul,Hiroaki Kawasaki,Hiroaki Kawasaki,Kazunari Taira,Kazunari Taira +10 more
TL;DR: A ribozyme library is used in an in vivo mouse model to identify genes related to metastasis that accelerated metastasis from pulmonary tumors that had developed from metastasizing cells andression of four of these by small interfering RNAs resulted in the accelerated mobility of cells in in vitro scratch-wound assay.
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Small interfering RNA expression vector targeting hypoxia-inducible factor 1 alpha inhibits tumor growth in hepatobiliary and pancreatic cancers
Takashi Mizuno,M Nagao,Yukishige Yamada,Michihiro Narikiyo,Masatou Ueno,Makoto Miyagishi,Kazunari Taira,Kazunari Taira,Yoshiyuki Nakajima +8 more
TL;DR: The usefulness of siRNA expression vector in targeting HIF1α is demonstrated and points to a potential clinical role in the treatment of pancreatic and hepatobiliary carcinomas.
Journal ArticleDOI
Identification of metastasis-related genes in a mouse model using a library of randomized ribozymes. VOLUME 279 (2004) PAGES 38083-38086
Eigo Suyama,Renu Wadhwa,Kamaljit Kaur,Makoto Miyagishi,Sunil C. Kaul,Hiroaki Kawasaki,Kazunari Taira +6 more
TL;DR: In this paper, the authors used a ribozyme library in an in vivo mouse model to identify genes related to metastasis, and injected weakly metastatic melanoma cells that had been treated with the library intravenously into mice.