M
Makoto Miyagishi
Researcher at National Institute of Advanced Industrial Science and Technology
Publications - 170
Citations - 19268
Makoto Miyagishi is an academic researcher from National Institute of Advanced Industrial Science and Technology. The author has contributed to research in topics: RNA interference & Small interfering RNA. The author has an hindex of 52, co-authored 161 publications receiving 18180 citations. Previous affiliations of Makoto Miyagishi include University of Tsukuba & Tohoku University.
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Journal ArticleDOI
Development and application of siRNA expression vector
Makoto Miyagishi,Kazunari Taira +1 more
TL;DR: A comparative analysis among various siRNA expression vectors is reported and a regulatable RNAi in cells is demonstrated by using a tetracycline-controlled U6 promoter.
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Transgenic small interfering RNA halts amyotrophic lateral sclerosis in a mouse model.
Yuki Saito,Takanori Yokota,Tasuku Mitani,Kaoru Ito,Masayuki Anzai,Makoto Miyagishi,Kazunari Taira,Hidehiro Mizusawa +7 more
TL;DR: It is proved that siRNA-mediated gene silencing can stop the development of familial ALS with SOD1 mutation by inhibiting mutant G93A S OD1 production in the central nervous system.
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Escape from the interferon response associated with RNA interference using vectors that encode long modified hairpin-RNA.
Hideo Akashi,Makoto Miyagishi,Makoto Miyagishi,Takanori Yokota,Tsunamasa Watanabe,Taro Hino,Kazutaka Nishina,Michinori Kohara,Kazunari Taira,Kazunari Taira +9 more
TL;DR: In this article, modified hairpin-RNAs (mhRNAs) of more than 100 bp, with multiple specific point-mutations within the sense strand and transcribed from the U6 or tRNAVal promoters, can cause RNAi without inducing the IFN pathway genes.
Journal ArticleDOI
siRNA-based inhibition specific for mutant SOD1 with single nucleotide alternation in familial ALS, compared with ribozyme and DNA enzyme
Takanori Yokota,Takanori Yokota,Makoto Miyagishi,Taro Hino,Ryusuke Matsumura,Tasinato Andrea,Makoto Urushitani,Rammohan V. Rao,Ryosuke Takahashi,Dale E. Bredesen,Kazunari Taira,Hidehiro Mizusawa +11 more
TL;DR: These siRNAs could suppress cell death of Neuro2a induced by over-expression of mutant SOD1s with stress of proteasome inhibition and support the feasibility of utilizing siRNA-based gene therapy of familial ALS with mutant S OD1.
Journal Article
Vectors for RNA interference.
TL;DR: This review describes the basis for, and methodology of siRNA expression vectors for mammalian cells that use polymerase III promoters, such as U6 or H1.