Manuela M. Pereira

TL;DR: A hypothesis is put forward to explain some apparently contradictory data on the nature of the coupling ion, and the role of H+ and Na+ cycles in the overall bioenergetics of the cell is revisited.

TL;DR: The hypothesis for E172 being of central importance in the catalytic mechanism of NDH-2, which may be extended to other members of the two-Dinucleotide Binding Domains Flavoprotein superfamily, is supported.

TL;DR: The results indicate that the transient interaction between Cyt-D and HiPIP relies upon a delicate balance of hydrophobic and polar contacts for establishing an optimized electron transfer pathway that involves the exposed edge of the heme group and guaranties efficient inter-protein electron transfer on the nanosecond time scale.

TL;DR: The open-reading-frames of the putative NhaD and PCD were shown to be co-transcribed with the other complex I genes encoded by nqoB, indicating the possible role of these two genes in R. marinus complex I.

TL;DR: The ligand-binding dynamics and the reaction with O(2) of the fully (five-electron) reduced cytochrome caa(3) from the thermohalophilic bacterium Rhodothermus (R.) marinus were investigated, finding the slower transition times as compared to those observed with the bovine enzyme most likely reflect the replacement of Glu(I-286) with a tyrosine in the R. marinus enzyme.