M
Marcel W. E. M. van Tilborg
Researcher at DSM
Publications - 3
Citations - 101
Marcel W. E. M. van Tilborg is an academic researcher from DSM. The author has contributed to research in topics: Fungal protein & Proteomics. The author has an hindex of 3, co-authored 3 publications receiving 94 citations.
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Journal ArticleDOI
Effective lead selection for improved protein production in Aspergillus niger based on integrated genomics
Denise Ilse Jacobs,Maurien M.A. Olsthoorn,Isabelle Maillet,Michiel Akeroyd,Stefaan Breestraat,Serge Petrus Donkers,Rob van der Hoeven,Cees A. M. J. J. van den Hondel,Rolf Kooistra,Thomas Lapointe,Hildegard Henna Menke,Rogier Meulenberg,Marijke Misset,Wally H. Müller,Noël Nicolaas Maria Elisabeth Van Peij,Arthur F. J. Ram,Sabrina Rodriguez,Marc S. Roelofs,Johannes Andries Roubos,Marcel W. E. M. van Tilborg,Arie J. Verkleij,Herman Jan Pel,Hein Stam,Cornelis Maria Jacobus Sagt +23 more
TL;DR: An integrated genomics approach was developed to determine cellular responses of A. niger to protein production in well-controlled fermentations and reduction of protein degradation through the removal of the ERAD factor doaA combined with overexpression of the oligosaccharyl transferase sttC resulted in a small increase in GUS expression.
Journal ArticleDOI
Nutrikinetic modeling reveals order of genistein phase II metabolites appearance in human plasma.
Suzanne Smit,Ewa Szymańska,Iris Kunz,Victoria Gomez Roldan,Marcel W. E. M. van Tilborg,Peter Weber,Kevin Prudence,Frans M. van der Kloet,John P. M. van Duynhoven,Age K. Smilde,Ric C. H. de Vos,Igor Bendik +11 more
TL;DR: The specific early appearance of G-4',7-diG suggests a multistep formation process for the mono and hetero genistein conjugates, involving one or two deglucuronidation steps.
Journal ArticleDOI
Searching for microbial protein over-expression in a complex matrix using automated high throughput MS-based proteomics tools.
Michiel Akeroyd,Maurien M.A. Olsthoorn,Jort Steven Johan Gerritsma,Diana Gutker-Vermaas,Laurens Ekkelkamp,Tjeerd van Rij,Paul Klaassen,Wim Plugge,Ed Smit,Kerstin Strupat,Thibaut José Wenzel,Marcel W. E. M. van Tilborg,Rob van der Hoeven +12 more
TL;DR: It is shown that this generic workflow can effectively reduce large expression libraries from fungi and bacteria to their minimal size by detection of successful protein over-expression using MS.