Maurien M.A. Olsthoorn

TL;DR: The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid, and the sequenced genome revealed a large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors.

TL;DR: The various strains, genetic techniques and molecular tools currently available for the use of K. lactis as a host for protein expression are reviewed and data illustrating the recent use of proteomics studies to identify cellular bottlenecks that impede heterologous protein expression is presented.

TL;DR: Comparison of transcript and protein levels clearly showed that upregulation in response to glucose limitation was mainly transcriptionally controlled, whereas upregulationIn response to nitrogen limitation was essentially controlled at the post‐transcriptional level by increased translational efficiency and/or decreased protein degradation.

TL;DR: It is shown here that the combined approach of chemostat cultivation and comprehensive proteome analysis allowed us to study the primary effect of single limiting conditions on the yeast proteome and unravel which processes in the central carbon metabolism were regulated at the level of the proteome, and which processes at thelevel of transcriptome.

TL;DR: An integrated genomics approach was developed to determine cellular responses of A. niger to protein production in well-controlled fermentations and reduction of protein degradation through the removal of the ERAD factor doaA combined with overexpression of the oligosaccharyl transferase sttC resulted in a small increase in GUS expression.