M
Marcelo Kern
Researcher at University of York
Publications - 6
Citations - 568
Marcelo Kern is an academic researcher from University of York. The author has contributed to research in topics: Cellulase & Opium Poppy. The author has an hindex of 5, co-authored 6 publications receiving 492 citations.
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Journal ArticleDOI
A Papaver somniferum 10-Gene Cluster for Synthesis of the Anticancer Alkaloid Noscapine
Thilo Winzer,Valeria Gazda,Zhesi He,Filip Kaminski,Marcelo Kern,Tony R. Larson,Yi Li,Fergus Meade,Roxana Teodor,Fabián E. Vaistij,Carol Walker,Tim Bowser,Ian A. Graham +12 more
TL;DR: In this paper, the exclusive expression of 10 genes encoding five distinct enzyme classes in a high noscapine-producing poppy variety, HN1, was found to be tightly linked in HN 1 and bacterial artificial chromosome sequencing confirmed that they exist as a complex gene cluster for plant alkaloids.
Journal ArticleDOI
Morphinan biosynthesis in opium poppy requires a P450-oxidoreductase fusion protein.
Thilo Winzer,Marcelo Kern,Andrew J. King,Tony R. Larson,Roxana Teodor,Samantha L. Donninger,Yi Li,Adam Dowle,Jared Cartwright,Rachel Bates,David A. Ashford,Jerry Thomas,Carol Walker,Tim Bowser,Ian A. Graham +14 more
TL;DR: The identification of an unusual protein that contains both cytochrome P-450 and oxidoreductase modules may enable microbial-based morphinan production and enable alternate routes for morphine biosynthesis that are less dependent on poppy cultivation.
Journal ArticleDOI
Structural characterization of a unique marine animal family 7 cellobiohydrolase suggests a mechanism of cellulase salt tolerance
Marcelo Kern,John McGeehan,Simon Streeter,Richard N. A. Martin,Katrin Besser,Luisa Elias,William Eborall,Graham P. Malyon,Christina M. Payne,Michael E. Himmel,Kirk Matthew Schnorr,Gregg T. Beckham,Simon M. Cragg,Neil C. Bruce,Simon J. McQueen-Mason +14 more
TL;DR: Overall, this study suggests that marine cellulases offer significant potential for utilization in high-solids industrial biomass conversion processes.
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Characterization of the cellulolytic secretome of Trichoderma harzianum during growth on sugarcane bagasse and analysis of the activity boosting effects of swollenin.
Vanessa Alves Lima Rocha,Roberto Nobuyuki Maeda,Nei Pereira,Marcelo Kern,Luisa Elias,Rachael Simister,Clare Steele-King,Leonardo D. Gomez,Simon J. McQueen-Mason +8 more
TL;DR: Despite lacking detectable glycoside activity, the addition of swollenin of T. harzianum increased by two‐fold the hydrolysis efficiency of a commercial cellulase cocktail, and the findings are of interest in enriching enzyme cocktail with this important accessory protein which takes part in the cellulose amorphogenesis.
Journal ArticleDOI
Hemocyanin Facilitates Lignocellulose Digestion by Wood-Boring Marine Crustaceans
Katrin Besser,Graham P. Malyon,William Eborall,Giovanni Paro da Cunha,Jefferson G. Filgueiras,Adam Dowle,Lourdes Cruz Garcia,Samuel J. Page,Ray Dupree,Marcelo Kern,Leonardo D. Gomez,Yi Li,Luisa Elias,Federico Sabbadin,Shaza Eva Mohamad,Shaza Eva Mohamad,Giovanna Pesante,Clare Steele-King,Eduardo Ribeiro de Azevêdo,Igor Polikarpov,Paul Dupree,Simon M. Cragg,Neil C. Bruce,Simon J. McQueen-Mason +23 more
TL;DR: Evidence is provided that the woodborer’s respiratory protein hemocyanin plays a central role in wood digestion and may offer a route toward biorefining of woody plant biomass.