Showing papers by "María Cristina Diez published in 2015"
TL;DR: The white-rot fungus Stereum hirsutum was studied to evaluate its applicability for use in the biosynthesis of copper/copper oxide nanoparticles under different pHconditions and in the presence of three different copper salts (CuCl2, CuSO4, and Cu(NO3)2) as discussed by the authors.
Abstract: The white-rot fungus Stereum hirsutum was studied to evaluate its applicability for use in the biosynthesis of copper/copper oxide nanoparticles under different pHconditions and in the presence of three different copper salts (CuCl2, CuSO4, and Cu(NO3)2). The nanoparticle formation was evaluated by UV-visible spectroscopy, electron microscopy (TEM), X-ray diffraction analysis (XRD), and Fourier transforms infrared spectroscopy (FTIR). The nanoparticles biosynthesis in presence of all copper salts demonstrated higher formation with 5 mM CuCl2 under alkaline conditions. TEM analysis confirmed that the nanoparticles were mainly spherical (5 to 20 nm). The presence of amine groups attached to nanoparticles was confirmed by FTIR, which suggests that extracellular protein of fungus is responsible for the formation of the nanoparticles. Therefore, the white-rot fungus S. hirsutum was found to exhibit potential for use in the synthesis of copper/copper oxide nanoparticles.
172 citations
TL;DR: The crop yield was negatively affected by all developed CRFs using biochar compared with the traditional fertilization, and the highest concentration of N in the leachates was the nitrate form.
142 citations
TL;DR: Evidence about the potential of white-rot fungi to degrade such pollutants as polycyclic aromatic hydrocarbons, dyes or antibiotics is discussed as an example of the complex structures that these microorganisms can attack.
Abstract: The use of white-rot fungi as a biotechnological tool for cleaning the environment of recalcitrant pollutants has been under evaluation for several years. However, it is still not possible to find sufficiently detailed investigations of this subject to conclude that these fungi can decontaminate the environment. In the present review, we have summarized and discussed evidence about the potential of white-rot fungi to degrade such pollutants as polycyclic aromatic hydrocarbons, dyes or antibiotics as an example of the complex structures that these microorganisms can attack. This review also discusses field experiment results and limitations of white-rot fungi trials from contaminated sites. Moreover, the use of catabolic potential of white-rot fungi in biopurification systems (biobeds) is also discussed. The current status and future perspectives of white-rot fungi, as a viable biotechnological alternative for improvement of environmental health are noted.
48 citations
TL;DR: FTIR results showed that amides, alkanes, carboxylates, car boxylates and hydroxyl groups may be important to the PCP adsorption for pellets of A. discolor, which may be used as a natural biosorbent for liquid solutions contaminated by PCP.
39 citations
TL;DR: This finding suggests that iprodione degradation by the isolate proceeds via the formation of intermediate metabolites before final hydrolysis to 3,5-DCA, a major dissipation process controlling its environmental dissipation.
33 citations
TL;DR: Evaluation of the diazinon removal achieved by the free actinobacteria and Streptomyces sp.
Abstract: Fil: Briceno, Gabriela. Universidad de la Frontera. Nucleo Cientifico y Tecnologico en Recursos Naturales; Chile;
26 citations
TL;DR: A method to selectively label sialoglycoproteins to identify new membrane and secreted proteins involved in the adaptative process of endothelial cells by mass spectrometry-based proteomics was used and validated.
19 citations
TL;DR: An increase in organic acid exudation by vegetal cover was observed caused by chemical stress after pesticide application, and was similar at both hydraulic loads.
Abstract: Interactions between microorganisms and root exudates in a biobed system with vegetal (grass) cover could enhance pesticide degradation. Otherwise, a high water load may generate high concentrations of pesticides in lixiviates. We studied the effect of the vegetal cover on the degradation of a mixture of atrazine (ATZ), chlorpyrifos (CHL) and iprodione (IPR) (35 mg L -1 each) in a biobed system operated with two different hydraulic loads (0.6 and 1.2 L of tap water per day). The concentration of the pesticides and their main metabolites were measured in the lixiviates during 60 days, as well as in the biomixtures at the end of the study. Dehydrogenase activity in the biomixtures and organic acid exudation from the vegetal cover were also analysed. The vegetal cover diminished the lixiviation of pesticides and their metabolites mainly at the lower hydraulic load used. The degradation of the pesticides was high (>95%) and increased in biobeds with vegetal cover and low hydraulic load. Degradation metabolites of CHL and IPR were formed during pesticide degradation; however they were degraded in the biobed and were not detected in lixiviates at the end of the study. In general, an increase in organic acid exudation by vegetal cover was observed caused by chemical stress after pesticide application. The increase was similar at both hydraulic loads. Efficient colonisation of wheat straw by fungi was observed by confocal microscopy.
15 citations
TL;DR: In this article, the effect of the rhizosphere of the grass layer on the dissipation of a combination of atrazine, chlorpyrifos and isoproturon was studied.
Abstract: In a biopurification system such as a biobed, the rhizosphere of the grass layer may be a significant factor for promoting pesticide dissipation in the biomixture. The rhizosphere effect of a Lolium perenne, Festuca arundinacea and Trifolium repens mixture on the dissipation of a pesticide combination that was composed of atrazine, chlorpyrifos and isoproturon was studied. The assay was performed using glass pots divided into two separate compartments (root surface and root-free), each filled with an organic biomixture (oat husk, top soil and peat) and contaminated with the pesticide mixture at 5 mg kg-1.Non-planted and non-contaminated pots were also used as controls. The results indicated that there were high atrazine, chlorpyrifos and isoproturon dissipation in the planted pots compared with the unplanted pots. An inverse correlation was found throughout the assay between phenoloxidase activity and residual pesticide (0.684 to 0.952). Indeed, fungal biomass was positively correlated with phenoloxidase activity on day 1 (r =0.825) and day 30 (r =0.855). Besides, exudation of oxalic and malic acid in contaminated pots was higher than in the control without pesticides, associated with oxidation of the pesticide mixture in the biomixture of a bioded system. Therefore, the grass layer enhances pesticide removal in biobeds.
13 citations
TL;DR: The effects of eight strains of white-rot fungi cultured on potato waste against the mycelial growth of Botrytis cinerea, Fusarium oxysporum and Mucor miehei were investigated using a bi-compartmented Petri dish assay.
Abstract: Fungal volatile secondary metabolites play important roles in mediating antagonistic and beneficial interactions among organisms. The effects of eight strains of white-rot fungi cultured on potato waste against the mycelial growth of Botrytis cinerea, Fusarium oxysporum and Mucor miehei were investigated using a bi-compartmented Petri dish assay. The chemical composition of volatile organic compounds released from the strain with the highest inhibitory effect was also investigated by headspace solid-phase microextraction and gas chromatography/mass spectrometry analysis. Of the eight white-rot fungi evaluated, Anthracophyllum discolor. Sp4 showed a high inhibitory activity against M. miehei (approximately 76%) and B. cinerea (approximately 20%). F. oxysporum was inhibited to a lesser extent (approximately 10%) by A. discolor and T. versiscolor. The gas chromatography/mass spectrometry analysis showed nine main volatile compounds released from A. discolor Sp4, among them are the sesquiterpenesα-bisabolene ...
13 citations