M
Mark Isalan
Researcher at Imperial College London
Publications - 102
Citations - 3964
Mark Isalan is an academic researcher from Imperial College London. The author has contributed to research in topics: Zinc finger & Gene. The author has an hindex of 31, co-authored 89 publications receiving 3540 citations. Previous affiliations of Mark Isalan include University of Cambridge & European Bioinformatics Institute.
Papers
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Journal ArticleDOI
A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter.
Mark Isalan,Aaron Klug,Yen Choo +2 more
TL;DR: A rapid and convenient method that can be used to design zinc finger proteins against a variety of DNA-binding sites and yields proteins that bind sequence-specifically to DNA with Kd values in the nanomolar range is presented.
Journal ArticleDOI
Evolvability and hierarchy in rewired bacterial gene networks
Mark Isalan,Caroline Lemerle,Konstantinos Michalodimitrakis,Carsten Horn,Pedro Beltrao,Emanuele Raineri,Mireia Garriga-Canut,Luis Serrano +7 more
TL;DR: It is shown that ∼95% of new networks are tolerated by the bacteria, that very few alter growth, and that expression level correlates with factor position in the wild-type network hierarchy, and therefore new links in the network are rarely a barrier for evolution and can even confer a fitness advantage.
Patent
Nucleic acid binding polypeptide library
Yen Choo,Aaron Klug,Mark Isalan +2 more
TL;DR: In this article, the authors describe a set of zinc finger polypeptide libraries which encode overlapping zinc fingers, with each polypide comprising more than one zinc finger which has been at least partially randomised.
Journal ArticleDOI
Synthetic zinc finger repressors reduce mutant huntingtin expression in the brain of R6/2 mice.
Mireia Garriga-Canut,Carmen Agustín-Pavón,Carmen Agustín-Pavón,Frank Herrmann,Frank Herrmann,Aurora Sánchez,Mara Dierssen,Cristina Fillat,Mark Isalan,Mark Isalan +9 more
TL;DR: Zinc finger repression was tested at several levels, resulting in protein aggregate reduction, reduced decline in rotarod performance, and alleviation of clasping in R6/2 mice, establishing a proof-of-principle for synthetic transcription factor repressors in the brain.
Journal ArticleDOI
Zinc-finger protein-targeted gene regulation: Genomewide single-gene specificity
Siyuan Tan,Dmitry Guschin,Albert R. Davalos,Ya-Li Lee,Andrew W. Snowden,Yann Jouvenot,H. Steven Zhang,Katherine Howes,Andrew R. McNamara,Albert Lai,Chris Ullman,Lindsey Reynolds,Michael J. Moore,Mark Isalan,Lutz-Peter Berg,Bradley Campos,Hong Qi,S. Kaye Spratt,Casey C. Case,Carl O. Pabo,Judith Campisi,Philip D. Gregory +21 more
TL;DR: It is reported that a ZFP TF can repress target gene expression with single-gene specificity within the human genome and was sufficient to generate a functional phenotype, as demonstrated by the loss of DNA damage-induced CHK2-dependent p53 phosphorylation.