Mark L. McCleland

TL;DR: In this article, a methodology was proposed to characterize most, if not all, phosphoproteins from a whole-cell lysate in a single experiment, and a total of 216 peptide sequences defining 383 sites of phosphorylation were determined.

TL;DR: These findings implicate Aurora B as a critical coordinator relating changes in microtubule dynamics in mitosis, chromosome movement in prometaphase and anaphase, signaling of the spindle checkpoint, and cytokinesis.

TL;DR: The data indicate that Aurora B kinase activity is regulated by both Survivin binding and cell cycle-dependent phosphorylation, and the hydrodynamic properties of the Aurora B/Survivin/INCENP complex are cell cycle regulated.

TL;DR: It is demonstrated that the spindle checkpoint can arrest budding yeast cells lacking NDC80 or Nuf2, whereas yeast lacking both proteins fail to arrest in mitosis, and proposed that the Ndc80 complex has conserved roles in kinetochore assembly, chromosome congression, and spindle checkpoints signaling.

TL;DR: Previous characterization of the Ndc80/Hec1 kinetochore complex is extended and it is found that Spc24 and Spc25 are required not only to establish, but also to maintain kinetchore-microtubule attachments and metaphase alignment.