M
Masaki Endo
Researcher at National Agriculture and Food Research Organization
Publications - 70
Citations - 3424
Masaki Endo is an academic researcher from National Agriculture and Food Research Organization. The author has contributed to research in topics: Gene & Cas9. The author has an hindex of 27, co-authored 65 publications receiving 2458 citations. Previous affiliations of Masaki Endo include University of Tsukuba & Kyoto University.
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Journal ArticleDOI
CRISPR/Cas9-mediated mutagenesis of the RIN locus that regulates tomato fruit ripening.
TL;DR: This study targeted the tomato RIN gene, which encodes a MADS-box transcription factor regulating fruit ripening, and targeted three regions within the gene, confirming the applicability of the CRISPR/Cas9 mutagenesis system in tomato.
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Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice
TL;DR: The approach presented here could be adapted to optimize the construction of Cas9/gRNA cassettes for genome editing in a variety of plants to improve the frequency of targeted mutagenesis in rice calli.
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Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice
TL;DR: To validate the applicability of the CRISPR/Cas9 system to target mutagenesis of paralogous genes in rice, a single-guide RNA (sgRNA) was designed that recognized 20 bp sequences of cyclin-dependent kinase B2 (CDKB2) as an on-target locus.
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Biallelic Gene Targeting in Rice.
TL;DR: There was no great difference in GT efficiency between Cas9-expressing and nonexpressing cells, however, when gRNA targeting DNA ligase 4 was transformed with Cas9 prior to the GT experiment, GT efficiency increased dramatically and more than one line exhibiting biallelic GT at the ALS locus was obtained.
Journal ArticleDOI
Re-evaluation of the rin mutation and the role of RIN in the induction of tomato ripening.
Yasuhiro Ito,Ayako Nishizawa-Yokoi,Masaki Endo,Masafumi Mikami,Yoko Shima,Nobutaka Nakamura,Eiichi Kotake-Nara,Susumu Kawasaki,Seiichi Toki,Seiichi Toki +9 more
TL;DR: Evidence is provided contradicting this concept of RIN function, showing induction of fruit ripening in the absence of Rin, and shows that the rin allele is a gain-of-function, rather than null, mutation.