M
Massimo Alfano
Researcher at Vita-Salute San Raffaele University
Publications - 118
Citations - 3299
Massimo Alfano is an academic researcher from Vita-Salute San Raffaele University. The author has contributed to research in topics: Viral replication & Chemokine. The author has an hindex of 29, co-authored 106 publications receiving 2823 citations.
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Journal ArticleDOI
Persistent Microbial Translocation and Immune Activation in HIV-1-Infected South Africans Receiving Combination Antiretroviral Therapy
Edana Cassol,Susan Malfeld,Phetole Walter Mahasha,Schalk W. van der Merwe,Sharon Cassol,Christopher J. Seebregts,Massimo Alfano,Guido Poli,Theresa M. Rossouw +8 more
TL;DR: The relationship between markers of monocyte activation, plasma lipopolysaccharide (LPS), and HIV-1 RNA in South Africans prioritized to receive combination antiretroviral therapy (cART) suggests that their upregulation was driven by HIV- 1.
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M1 and M2a polarization of human monocyte-derived macrophages inhibits HIV-1 replication by distinct mechanisms.
TL;DR: It is demonstrated that cytokine-induced polarization of human monocyte-derived macrophage into either classical (M1) or alternatively activated (M2a) MDM is associated with a reduced capacity to support productive CCR5-dependent HIV-1 infection.
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Biological and Technical Variables Affecting Immunoassay Recovery of Cytokines from Human Serum and Simulated Vaginal Fluid: A Multicenter Study
Raina N. Fichorova,Nicola Richardson-Harman,Massimo Alfano,Laurent Bélec,Cédric Carbonneil,Silvia Chen,Lisa A. Cosentino,Kelly A. Curtis,Charlene S. Dezzutti,Betty A. Donoval,Gustavo F. Doncel,Melissa Donaghay,Jean-Charles Grivel,Esmeralda Guzman,Madeleine Hayes,Betsy C. Herold,Sharon L. Hillier,Carol Lackman-Smith,Alan L. Landay,Leonid Margolis,Kenneth H. Mayer,Jenna Malia Pasicznyk,Melanie Pallansch-Cokonis,Guido Poli,Patricia Reichelderfer,Paula Roberts,Irma Rodriguez,Héla Saïdi,Rosaria Rita Sassi,Robin Shattock,James E. Cummins +30 more
TL;DR: The results suggest the need for uniform vaginal sampling techniques and a better understanding of immunoassay platform differences and cross-validation before the biological significance of cytokine variations can be validated in clinical trials.
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Human immunodeficiency virus type 1 T-lymphotropic strains enter macrophages via a CD4- and CXCR4-mediated pathway: replication is restricted at a postentry level.
TL;DR: It is suggested that postentry steps are critical for restricted replication of T-lymphotropic HIV-1 strains in macrophages.
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Macrophage polarization and HIV-1 infection
TL;DR: In vitro polarization into M1 cells prevents HIV‐1 infection, and M2a polarization inhibits viral replication at a post‐integration level, and in vivo polarization of MP is likely to span a spectrum of activation phenotypes that may change the permissivity to and alter the outcome of HIV‐ 1 and other viral infections.