Melina Theoni Gyparaki

TL;DR: This Primer explains the central concepts of single-molecule localization microscopy before discussing experimental considerations regarding fluorophores, optics and data acquisition, processing and analysis, and describes recent high-impact discoveries made by SMLM techniques.

TL;DR: It is established that RAD52 and SLX4 mediate distinct postreplicative DNA repair processes that maintain ALT telomere stability and cancer cell viability.

TL;DR: In this article, the spatial distribution of tau monomers and oligomers inside cells under physiological or pathological conditions has not been visualized, and the authors showed that tau forms small oligomers on microtubules ex vivo.

TL;DR: This chapter describes several methodologies to delineate major steps of HDR during alternative lengthening of telomeres in human cells, including procedures to visualize interchromosomal telomere homology searches in real time and quantitatively detect HDR synthesis of nascent Telomere DNA double-strand breaks.

TL;DR: It is shown that, in vivo, tau forms small oligomers on microtubules under physiological conditions, which elucidates tau’s nanoscale composition under physiological and pathological conditions in vivo.