M
Mickaël Lelek
Researcher at Pasteur Institute
Publications - 24
Citations - 2146
Mickaël Lelek is an academic researcher from Pasteur Institute. The author has contributed to research in topics: Microscopy & Live cell imaging. The author has an hindex of 13, co-authored 21 publications receiving 1633 citations. Previous affiliations of Mickaël Lelek include Centre national de la recherche scientifique & Sorbonne.
Papers
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Journal ArticleDOI
QuickPALM: 3D real-time photoactivation nanoscopy image processing in ImageJ
Ricardo Henriques,Ricardo Henriques,Mickaël Lelek,Eugenio F. Fornasiero,Flavia Valtorta,Christophe Zimmer,Musa M. Mhlanga,Musa M. Mhlanga +7 more
TL;DR: To the Editor: Although conventional microscopes have a reso-lution limited by diffraction to about half the wavelength of light, several recent advances have led to microscopy methods that achieve roughly tenfold improvements in resolution.
Journal ArticleDOI
Deep learning massively accelerates super-resolution localization microscopy
Wei Ouyang,Wei Ouyang,Andrey Aristov,Andrey Aristov,Mickaël Lelek,Mickaël Lelek,Xian Hao,Xian Hao,Christophe Zimmer,Christophe Zimmer +9 more
TL;DR: Simulations and experimental imaging of microtubules, nuclear pores, and mitochondria show that high-quality, super-resolution images can be reconstructed from up to two orders of magnitude fewer frames than usually needed, without compromising spatial resolution.
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Single-molecule localization microscopy
Mickaël Lelek,Mickaël Lelek,Melina Theoni Gyparaki,Gerti Beliu,Florian Schueder,Florian Schueder,Juliette Griffié,Suliana Manley,Ralf Jungmann,Ralf Jungmann,Markus Sauer,Melike Lakadamyali,Christophe Zimmer,Christophe Zimmer +13 more
TL;DR: This Primer explains the central concepts of single-molecule localization microscopy before discussing experimental considerations regarding fluorophores, optics and data acquisition, processing and analysis, and describes recent high-impact discoveries made by SMLM techniques.
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Entrapment of Intracytosolic Bacteria by Septin Cage-like Structures
Serge Mostowy,Serge Mostowy,Serge Mostowy,Matteo Bonazzi,Matteo Bonazzi,Matteo Bonazzi,Melanie Hamon,Melanie Hamon,Melanie Hamon,To Nam Tham,To Nam Tham,To Nam Tham,Adeline Mallet,Mickaël Lelek,Mickaël Lelek,Edith Gouin,Edith Gouin,Edith Gouin,Caroline Demangel,Roland Brosch,Christophe Zimmer,Christophe Zimmer,Anna Sartori,Makoto Kinoshita,Marc Lecuit,Marc Lecuit,Marc Lecuit,Pascale Cossart,Pascale Cossart,Pascale Cossart +29 more
TL;DR: It is reported that septin assemblies are recruited to different bacteria that polymerize actin, and it is observed that intracytosolic Shigella either become compartmentalized in septin cage-like structures or form actin tails.
Journal ArticleDOI
Superresolution imaging of HIV in infected cells with FlAsH-PALM
Mickaël Lelek,Francesca Di Nunzio,Ricardo Henriques,Pierre Charneau,Nathalie J. Arhel,Christophe Zimmer +5 more
TL;DR: The application of FlAsH-PALM to image the integrase enzyme (IN) of HIV in fixed and living cells under experimental conditions that fully preserved HIV infectivity is applied.