M
Melissa Hardy
Researcher at University of Utah
Publications - 5
Citations - 1915
Melissa Hardy is an academic researcher from University of Utah. The author has contributed to research in topics: Slit & Zebrafish. The author has an hindex of 5, co-authored 5 publications receiving 1686 citations.
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Journal ArticleDOI
The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs.
Kristen M. Kwan,Esther Fujimoto,Clemens Grabher,Benjamin D. Mangum,Melissa Hardy,Douglas Simon Campbell,John M. Parant,H. Joseph Yost,John P. Kanki,Chi Bin Chien +9 more
TL;DR: The Tol2kit greatly facilitates zebrafish transgenesis, simplifies the sharing of clones, and enables large‐scale projects testing the functions of libraries of regulatory or coding sequences.
Journal ArticleDOI
Axon tracking in serial block-face scanning electron microscopy
Elizabeth Jurrus,Melissa Hardy,Tolga Tasdizen,P. Thomas Fletcher,Pavel Koshevoy,Chi-Bin Chien,Winfried Denk,Ross T. Whitaker +7 more
TL;DR: A carefully engineered algorithm using Kalman-snakes and optical flow computation is presented and results indicate that this algorithm can significantly speed up the task of manual axon tracking.
Journal ArticleDOI
Generating X: formation of the optic chiasm.
TL;DR: Whether axons cross is instead controlled by the transcription factor Zic2 and the guidance receptor EphB1, as shown by the current issues of Neuron and Cell.
Journal ArticleDOI
Focal Gene Misexpression in Zebrafish Embryos Induced by Local Heat Shock Using a Modified Soldering Iron
TL;DR: A new method of misexpression: local heat shock using a modified soldering iron for zebrafish carrying transgenes under the control of a heat shock promoter (hsp70) is described.
Journal ArticleDOI
Analysis of the astray/robo2 Zebrafish Mutant Reveals that Degenerating Tracts Do Not Provide Strong Guidance Cues for Regenerating Optic Axons
Cameron Wyatt,Anselm Ebert,Michell M. Reimer,Kendall Rasband,Melissa Hardy,Chi-Bin Chien,Thomas Becker,Catherina G. Becker +7 more
TL;DR: There is not an efficient correction mechanism for large-scale pathfinding errors of optic axons during development, degenerating tracts do not provide a strong guidance cue for regenerating optic axon in the adult CNS, unlike the PNS, and robo2 is less important for pathfinding of opticAxons during regeneration than during development.