Meredith N Frazier

TL;DR: In this paper, the authors used cryo-EM to capture structures of Nsp15 bound to RNA in pre-and post-cleavage states, and determined how the sequence of the RNA substrate dictates cleavage and found that outside of polyU tracts, nsp15 has a strong preference for purines 3' of the cleaved uridine.

TL;DR: Recent structures revealing molecular insight into higher order enzymatic assemblies are summarized with an emphasis on the interplay between discrete active sites.

TL;DR: In this paper, a series of cryo-EM reconstructions of SARS-CoV-2 Nsp15, in both apo and UTP-bound states, are presented.

TL;DR: A series of cryo-EM reconstructions of SARS-CoV-2 Nsp15 advance understanding of how NSP15 processes viral RNA and provide a structural framework for the development of new therapeutics.

TL;DR: In this article, the authors used cryo-EM to capture structures of Nsp15 bound to RNA in pre-and post-cleavage states, and determined how the sequence of the RNA substrate dictates cleavage and found that outside of polyU tracts, Nsp 15 has a strong preference for purines 3 of the cleaved uridine.