Mi Sook Chang

TL;DR: These results provide an alternative strategy for eliciting RNAi-mediated target cleavage using low concentrations of synthetic RNA as substrates for cellular Dicer-mediated cleavage.

TL;DR: Key anti-inflammatory effects of cAMP were mediated specifically by cAMP-dependent protein kinase (PKA), and selective inhibitors of PKA anchoring, time-lapse microscopy, and RNAi screening suggest that crosstalk between TLR4 and cAMP pathways in macrophages can be coordinated through PKA-dependent scaffolds that localize specific pools of the kinase to distinct substrates.

TL;DR: A comparison of several microarray platforms as well as analysis of various methods used for microarray target preparation and the reference design indicate that the pairwise correlations of expression levels between platforms are relative low overall but that the log ratios of the highly expressed genes are strongly correlated, especially between Affymetrix and cDNA arrays.

TL;DR: A flexible platform using RNA polymerase II promoter-driven expression of microRNA-like short hairpin RNAs which permits robust depletion of multiple target genes from a single transcript and highlights the versatility of this vector platform for multiple target gene knockdown in mammalian cells.

TL;DR: A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response.