M
Micaela C. Damsten
Researcher at VU University Amsterdam
Publications - 6
Citations - 351
Micaela C. Damsten is an academic researcher from VU University Amsterdam. The author has contributed to research in topics: Cytochrome P450 & Serum albumin. The author has an hindex of 6, co-authored 6 publications receiving 335 citations.
Papers
More filters
Journal ArticleDOI
Heterotropic and homotropic cooperativity by a drug-metabolising mutant of cytochrome P450 BM3.
Barbara M A van Vugt-Lussenburg,Micaela C. Damsten,Dianne M. Maasdijk,Nico P. E. Vermeulen,Jan N. M. Commandeur +4 more
TL;DR: The mutant of the bacterial cytochrome P450 BM3 is able to metabolise testosterone and several drug-like molecules such as amodiaquine, dextromethorphan, acetaminophen, and 3,4-methylenedioxymethylamphetamine that are known substrates of human P450s.
Journal ArticleDOI
Application of drug metabolising mutants of cytochrome P450 BM3 (CYP102A1) as biocatalysts for the generation of reactive metabolites.
Micaela C. Damsten,Barbara M A van Vugt-Lussenburg,Tineke Zeldenthuis,Jon S.B. de Vlieger,Jan N. M. Commandeur,Nico P. E. Vermeulen +5 more
TL;DR: It was shown that the four CYP102A1 mutants used in this study formed the same metabolites as human and rat liver microsomes, with an activity up to 70-fold higher compared to human enzymes.
Journal ArticleDOI
Liquid Chromatography/Tandem Mass Spectrometry Detection of Covalent Binding of Acetaminophen to Human Serum Albumin
Micaela C. Damsten,Jan N. M. Commandeur,A. Fidder,Albert G. Hulst,Daan J Touw,Daan Noort,Nico P. E. Vermeulen +6 more
TL;DR: This strategy could be seen as a potential biomonitoring tool to detect in vivo reactive intermediates of drugs and drug candidates, e.g., in the preclinical and clinical development phase.
Journal ArticleDOI
Trimethoprim: Novel Reactive Intermediates and Bioactivation Pathways by Cytochrome P450s
Micaela C. Damsten,Jon S.B. de Vlieger,Wilfried M. A. Niessen,Hubertus Irth,Nico P. E. Vermeulen,Jan N. M. Commandeur +5 more
TL;DR: Preliminary results might implicate that genetic polymorphisms in P450 enzymes could play a role in the onset of TMP-related ADRs in humans.
Journal ArticleDOI
Bioactivation of dibrominated biphenyls by cytochrome P450 activity to metabolites with estrogenic activity and estrogen sulfotransferase inhibition capacity
Marola M. H. Van Lipzig,Jan N. M. Commandeur,Frans J. J. de Kanter,Micaela C. Damsten,Nico P. E. Vermeulen,Evelina Maat,E.J. Groot,Abraham Brouwer,Monique H. A. Kester,Theo J. Visser,John H. N. Meerman +10 more
TL;DR: It is demonstrated that two dibrominated biphenyls are bioactivated by CYP activity into very potent estrogenic metabolites and inhibitors of hEST.