M
Michael H.L. Green
Researcher at University of Brighton
Publications - 130
Citations - 6470
Michael H.L. Green is an academic researcher from University of Brighton. The author has contributed to research in topics: DNA damage & Comet assay. The author has an hindex of 43, co-authored 130 publications receiving 6383 citations. Previous affiliations of Michael H.L. Green include University of Sussex.
Papers
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Journal ArticleDOI
The single cell gel electrophoresis assay (comet assay): A European review
Valerie J. McKelvey-Martin,Michael H.L. Green,P. Schmezer,Beatrice L. Pool-Zobel,M.P. De Méo,Andrew Collins +5 more
TL;DR: The development of the SCGE assay, existing protocols for the detection and analysis of comets, the relevant underlying principles determining the behaviour of DNA, and the potential applications of the technique are reviewed.
Journal ArticleDOI
Mutagen testing using TRP+ reversion in Escherichia coli.
Michael H.L. Green,W.J. Muriel +1 more
TL;DR: Escherichia coli strain WP2 and its repair-deficient derivatives are suitable strains for mutagen screening because agents which cause base substitution mutations can be shown to increase the frequency of Trp+ revertants.
Journal ArticleDOI
Use of a simplified fluctuation test to detect low levels of mutagens
TL;DR: A modification of the Luria and Delbrück fluctuation test in which the individual tubes are scored by eye for the presence or absence of a mutation appears to induce more mutations than might be predicted by extrapolation from short exposure experiments at higher doses.
Journal ArticleDOI
UV-C sensitivity of unstimulated and stimulated human lymphocytes from normal and xeroderma pigmentosum donors in the comet assay: a potential diagnostic technique.
Michael H.L. Green,Jillian E. Lowe,Susan A. Harcourt,P. Akinluyi,T. Rowe,Jane Cole,Alexander Vincent Anstey,Colin F. Arlett +7 more
TL;DR: Few strand breaks were seen in either unstimulated or stimulated lymphocytes of four xeroderma pigmentosum donors, suggesting that the comet (single-cell microgel electrophoresis) assay may offer a rapid diagnostic assay for XP.
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Endogenous nitric oxide induced by interleukin-1β in rat islets of Langerhans and HIT-T15 cells causes significant DNA damage as measured by the ‘comet’ assay
TL;DR: Intracellular levels of nitric oxide generated by interleukin‐1β‐induced nitricoxide synthase were sufficient to cause DNA damage in islet cells and HIT‐T15 cells, suggesting thatNitric oxide itself, rather than Superoxide or peroxynitrite may be the active species.