M
Michael J. Francis
Researcher at Wellcome Trust Centre for Human Genetics
Publications - 19
Citations - 743
Michael J. Francis is an academic researcher from Wellcome Trust Centre for Human Genetics. The author has contributed to research in topics: Menkes disease & Gene mapping. The author has an hindex of 15, co-authored 19 publications receiving 731 citations. Previous affiliations of Michael J. Francis include University of Oxford & Wellcome Trust.
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Journal ArticleDOI
A Golgi Localization Signal Identified in the Menkes Recombinant Protein
Michael J. Francis,Emma E. Jones,Elaine R. Levy,Sreenivasan Ponnambalam,Jamel Chelly,Anthony P. Monaco +5 more
TL;DR: The full-length recombinant Menkes protein was shown by immunofluorescence to localize to the Golgi apparatus and the alternatively spliced form, lacking sequences for transmembrane domains 3 and 4 encoded by exon 10, was shown to localizing to the endoplasmic reticulum.
Journal ArticleDOI
Identification of a di-leucine motif within the C terminus domain of the Menkes disease protein that mediates endocytosis from the plasma membrane.
Michael J. Francis,Emma E. Jones,Elaine R. Levy,Rowena L. Martin,Sreenivasan Ponnambalam,Anthony P. Monaco +5 more
TL;DR: A di-leucine motif (L1487L1488) was essential for rapid internalisation of chimeric CD8 proteins and the full-length Menkes cDNA from the plasma membrane, and it is suggested that this motif mediates the retrieval of MNK from the Plasma membrane into the endocytic pathway, via the recycling endosomes, but is not sufficient to return the protein to the Golgi apparatus.
Journal ArticleDOI
A pH-sensitive fluor, CypHer 5, used to monitor agonist-induced G protein-coupled receptor internalization in live cells.
Elaine Adie,Kalinka S,Lynne Smith,Michael J. Francis,A Marenghi,Michael Edward Cooper,Mark Samuel Jonathan Briggs,N P Michael,G Milligan,Game S +9 more
TL;DR: The red fluorescence of CypHer 5 provides a generic reagent suitable for monitoring the internalization of GPCRs into acidic vesicles, and should be amenable to the study of many other classes of cell surface receptors that also internalize following stimulation.
Journal ArticleDOI
Characterization of the Menkes Protein Copper-Binding Domains and Their Role in Copper-Induced Protein Relocalization
TL;DR: The copper-binding domains are vital for MNK trafficking and only a single domain is sufficient for this redistribution to occur.
Journal ArticleDOI
A contig of non-chimaeric YACs containing the spinal muscular atrophy gene in 5q13
Michael J. Francis,Karen E. Morrison,L Campbell,Prabhjit K. Grewal,Zoe Christodoulou,R. J. Daniels,Anthony P. Monaco,Anne Marie Frischauf,John Douglas Mcpherson,John J. Wasmuth,Kay E. Davies +10 more
TL;DR: The data show the value of screening several YAC libraries simultaneously in order to construct a set of overlapping sequences suitable for candidate gene searches and direct genomic sequencing.