Michael M. Meagher

TL;DR: In this article, room temperature ionic liquids have potential as extractants for butyl alcohol extraction from fermentation broth; water solubility in ionic liquid and ionic Liquid Solubility (LSL) in water are important factors for selectivity of butyl Alcohol extraction from aqueous solutions.

TL;DR: The information in this chapter should enable a reader with little or no experience to perform a high-cell density fermentation of a P. pastoris expression strain to achieve high expression levels with almost any good-quality fermentor, modified to accommodate this organism.

TL;DR: In this article, a silicalite-silicon composite membrane (306μm thick) was made in a laboratory and characterized for flux and selectivities using model acetone ethanol butanol solution.

TL;DR: An unstructured growth model for the recombinant methylotrophic yeast P. pastoris Mut(+) expressing the heavy-chain fragment C of botulinum neurotoxin serotype A [BoNT/A(H(c))], was successfully established in quasi-steady state fed-batch fermentations with varying cell densities.

TL;DR: The results show that either one of alanine, sorbitol, mannitol or trehalose can be used as a sole carbon and energy source for P. pastoris, although the doubling time on tre Halose was very long.