Journal ArticleDOI
Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris.
Mehmet Inan,Michael M. Meagher +1 more
TLDR
The results show that either one of alanine, sorbitol, mannitol or trehalose can be used as a sole carbon and energy source for P. pastoris, although the doubling time on tre Halose was very long.About:
This article is published in Journal of Bioscience and Bioengineering.The article was published on 2001-01-01. It has received 183 citations till now. The article focuses on the topics: Pichia pastoris & Sorbitol.read more
Citations
More filters
Journal ArticleDOI
Heterologous protein production using the Pichia pastoris expression system.
TL;DR: The Pichia pastoris expression system is being used successfully for the production of various recombinant heterologous proteins and the importance of optimizing the physicochemical environment for efficient and maximal recombinant protein production in bioreactors and the role of process control in optimizing protein production is reviewed.
Journal ArticleDOI
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
Rachel Daly,Milton T.W. Hearn +1 more
TL;DR: The purpose of this review is to summarize important developments and features of this expression system and to examine from an experimental perspective the genetic engineering, protein chemical and molecular design considerations that have to be taken into account for the successful expression of the target recombinant protein.
Journal ArticleDOI
Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production.
TL;DR: This review refers to established tools in protein expression in P. pastoris and highlights novel developments in the areas of expression vector design, host strain engineering and screening for high-level expression strains.
Journal ArticleDOI
Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
TL;DR: The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression system for heterologous protein production and the use of mixed substrates, on-line monitoring of the key fermentation parameters (methanol) and control algorithms applied to the bioprocess are reviewed and discussed in detail.
Journal ArticleDOI
Cultivation strategies to enhance productivity of Pichia pastoris: A review
Verena Looser,Béla Brühlmann,Fabian Bumbak,Chantal Stenger,Merixtell Costa,Andrea Camattari,Dimitrios Fotiadis,Karin Kovar +7 more
TL;DR: This review focuses on particular aspects of recombinant protein production related to variations in biomass growth and their implications for strain design and screening, as well as on the concept of rational comparisons between cultivation systems for the development of specific production processes in bioreactors.
References
More filters
Book
Experiments in molecular genetics
TL;DR: Molecular Genetics (Biology): An Overview | Sciencing Experimental in Molecular Genetics Experiments in molecular genetics (1972 edition) | Open ...
Journal ArticleDOI
Foreign gene expression in yeast: a review.
TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.
Journal ArticleDOI
Recent advances in the expression of foreign genes in Pichia pastoris
TL;DR: The Pichia pastoris heterologous gene expression system has been utilized to produce attractive levels of a variety of intracellular and extracellular proteins of interest and improvements in understanding and application have improved its utility even further.
Journal ArticleDOI
Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris
Koti Sreekrishna,Robert G. Brankamp,Keith E. Kropp,Dale T. Blankenship,Jiu-Tsair Tsay,Phil L. Smith,Jonathan D. Wierschke,A Subramaniam,Lori A. Birkenberger +8 more
TL;DR: The inherent ability of P. pastoris to convert the zymogen (pro-enzyme) form of matrix metalloproteinases (MMP) into active mature forms (which tend to self-degrade, and in some instances also cause damage to cells), largely limits the use of this system for the production of MMP, but this problem can be partly alleviated by co-expression of tissue inhibitor of M MP (TIMP-1).
Journal ArticleDOI
Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae
Leonard Guarente,Mark Ptashne +1 more
TL;DR: An analysis of deletions in the upstream DNA suggests that sequences required for efficient transcription initiation of CYC1 lie within the DNA segment 250--700 base pairs upstream from the start of the CYC 1 coding sequence.