Mehmet Inan

TL;DR: The results show that either one of alanine, sorbitol, mannitol or trehalose can be used as a sole carbon and energy source for P. pastoris, although the doubling time on tre Halose was very long.

TL;DR: It can be concluded that methanol metabolism along with cell lysis towards the end of fermentation contributes to increased proteolytic activity and eventual degradation of recombinant protein.

TL;DR: Overexpression of the endoplasmic reticulum resident, homologous chaperone protein, protein disulfide isomerase (PDI) was able to increase the secretion of (Na‐ASP1) protein in high copy clones, and increase in secreted Na‐ ASP1 secretion is correlated well with the PDI copy number.

TL;DR: Despite the inability of the human host to develop naturally acquired immune responses to hookworm, there is evidence for the feasibility of developing a vaccine based on the successes of immunising laboratory animals with either attenuated larval vaccines or antigens extracted from the alimentary canal of adult blood-feeding stages

TL;DR: Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are investigated and characteristics of the expression system, such as phenotypes and carbon utilization, are summarized.