M
Miranda Gibson
Researcher at University of Kentucky
Publications - 6
Citations - 309
Miranda Gibson is an academic researcher from University of Kentucky. The author has contributed to research in topics: Aglycone & Gene. The author has an hindex of 6, co-authored 6 publications receiving 294 citations.
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Journal ArticleDOI
Characterization of kinetics and products of the Baeyer-Villiger oxygenase MtmOIV, the key enzyme of the biosynthetic pathway toward the natural product anticancer drug mithramycin from Streptomyces argillaceus.
TL;DR: MtmOIV, the key oxygenase of the mithramycin biosynthetic pathway in Streptomyces argillaceus, was proven to act initially as Baeyer-Villiger monooxygenase, but may also catalyze various follow-up reaction steps.
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Engineering Biosynthetic Pathways for Deoxysugars: Branched-Chain Sugar Pathways and Derivatives from the Antitumor Tetracenomycin
Felipe Lombó,Miranda Gibson,Lisa Greenwell,Alfredo F. Braña,Jürgen Rohr,José A. Salas,Carmen Méndez +6 more
TL;DR: Formation of two compounds represents examples of the sugar cosubstrate flexibility of the ElmGT glycosyltransferase, and provided insights into the substrate flexibility of deoxysugar biosynthesis enzymes as the C-methyltransferases EryBIII and MtmC, the epimerases OleL and EryBVII, and the 4-ketoreductases ERYV and OleU.
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Combining sugar biosynthesis genes for the generation of L- and D-amicetose and formation of two novel antitumor tetracenomycins.
María Esther del Moral Pérez,Felipe Lombó,Lili Zhu,Miranda Gibson,Alfredo F. Braña,Jürgen Rohr,José A. Salas,Carmen Méndez +7 more
TL;DR: L- and D-stereoisomers of amicetose were generated by combining sugar biosynthesis genes from four different antibiotic gene clusters and both sugars were transferred to the elloramycin aglycone by the sugar flexible ElmGT glycosyltransferase.
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Generation of Novel Landomycins M and O through Targeted Gene Disruption
Andriy Luzhetskyy,Lili Zhu,Miranda Gibson,Marta Fedoryshyn,Clemens Dürr,Carsten Hofmann,Dirk Hoffmeister,Bohdan Ostash,Cynthia Mattingly,Val R. Adams,Victor Fedorenko,Jürgen Rohr,Andreas Bechthold +12 more
TL;DR: It is shown that LanZ4 and LanZ5 are responsible for the unique C‐11‐hydroxylation that occurs during landomycin biosynthesis.
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Crystallization and X‐ray diffraction properties of Baeyer–Villiger monooxygenase MtmOIV from the mithramycin biosynthetic pathway in Streptomyces argillaceus
TL;DR: Structural solution combining molecular-replacement phases and anomalous phases from selenium is in progress, and L-Selenomethionine-incorporated MtmOIV has been obtained.