M
Mithra Korukonda
Publications - 5
Citations - 54
Mithra Korukonda is an academic researcher. The author has contributed to research in topics: Biology & Gene. The author has an hindex of 2, co-authored 5 publications receiving 54 citations.
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Journal ArticleDOI
High-plex imaging of RNA and proteins at subcellular resolution in fixed tissue by spatial molecular imaging.
Shanshan He,Ruchir Bhatt,Carl O. Brown,Emily A. Brown,Derek L. Buhr,Kan Chantranuvatana,Patrick Danaher,Dwayne Dunaway,Ryan G. Garrison,Gary K. Geiss,Mark Gregory,Margaret L. Hoang,Rustem Khafizov,Emily E. Killingbeck,Dae Kim,Tae-Kyung Kim,Youngmi Kim,Andrew Klock,Mithra Korukonda,Alecksandr J. Kutchma,Zachary R. Lewis,Yang Xiang,J. Stuart Nelson,Giang T. Ong,Evan P. Perillo,Joseph C. Phan,Tien Phan-Everson,Erin Piazza,Tushar A. Rane,Zachary Reitz,M. Rhodes,Alyssa B. Rosenbloom,D. Ross,Hiromi Sato,Aster Wardhani,Corey A. Williams-Wietzikoski,Lidan Wu,Joseph M. Beechem +37 more
TL;DR: S spatial molecular imaging is described, a system that measures RNAs and proteins in intact biological samples at subcellular resolution by performing multiple cycles of nucleic acid hybridization of fluorescent molecular barcodes, and has high sensitivity and very low error rate.
Journal ArticleDOI
Abstract 3878: Subcellular characterization of over 100 proteins in FFPE tumor biopsies with CosMx Spatial Molecular Imager
Zachary R. Lewis,Tien Phan-Everson,Gary K. Geiss,Mithra Korukonda,Ruchir Bhatt,Carl O. Brown,Dwayne Dunaway,Joseph C. Phan,Alyssa B. Rosenbloom,Brian Filanoski,Rhonda Meredith,Kan Chantranuvatana,Yan Liang,E H Brown,Brian Birditt,Giang T. Ong,Hyeri Yi,Erin Piazza,Vikram Devgan,Nicole Ortogero,Patrick Alan Danaher,Sarah Warren,Michael Craig Rhodes,Joseph M. Beechem +23 more
TL;DR: The CosMx 100-plex immuno-oncology assay can be coupled with SMI’s 1000-plex RNA-detection assay and generate an unprecedented information-rich view of spatial biology that could usher in novel discoveries about health and disease.
Journal ArticleDOI
Abstract 4617: A complete pipeline for high-plex spatial proteomic profiling and analysis on the cosmxtm spatial molecular imager and atomtm spatial informatics platform
Tien Phan-Everson,Zachary R. Lewis,Giang T. Ong,Yang Xiang,Emily Brown,Liuliu Pan,Aster Wardhani,Mithra Korukonda,Carl O. Brown,Dwayne Dunaway,Edward Zhao,Sangsoon Woo,Alyssa B. Rosenbloom,Brian Filanoski,Rhonda Meredith,Kan Chantranuvatana,Brian Birditt,Hyeri Yi,Erin Piazza,Jason Reeves,John S. Lyssand,Vik Devgan,Michael Rhodes,Gary K. Geiss,Joseph M. Beechem +24 more
TL;DR: The AtoMx spatial molecular imager (SMI) as discussed by the authors is a high-level spatial multi-omics platform that enables detection of more than 110 proteins at subcellular resolution in real-world FFPE tissues.
Proceedings ArticleDOI
147 Comprehensivein situimmune phenotyping with the CosMx™ high plex protein assay
Tien Phan-Everson,Jianji Chen,Zachary R. Lewis,Gary K. Geiss,Yang Xiang,Emily Brown,Liuliu Pan,Stefan J. Phelan,Charlie Glaser,Mithra Korukonda,Carl O. Brown,Dwayne Dunaway,Joseph C. Phan,Alyssa B. Rosenbloom,Brian Filanoski,Rhonda Meredith,Kan Chantranuvatana,Brian Birditt,Giang T. Ong,Hyeri Yi,Erin Piazza,Jason Reeves,Subham Basu,Christine H Kang,Vikram Devgan,Edward Zhao,M. Rhodes,Joseph M. Beechem +27 more
TL;DR: The CosMx SMI platform as mentioned in this paper uses antibodies conjugated with oligonucleotides, which are detected using universal, multi-analyte cosMx readout reagents.
Journal ArticleDOI
Abstract 5641: Single cell spatial molecular imaging of 76-plex proteins in clinical cancer samples in response to personalized treatment
Zachary R. Lewis,Brian Birditt,Emily Brown,Kan Chantranuvatana,Brian Filanoski,Christopher L. Corless,Tien Phan-Everson,Gary K. Geiss,Tyler Hether,Evie Hobbs,Brett Johnson,Taylor Kelley,Rhonda Meredith,Anastasiya Olson,Giang T. Ong,Mithra Korukonda,Erin Piazza,Jason Reeves,Alyssa B. Rosenbloom,Kiara Siex,Hyeri Yi,Edward Zhao,Joseph M. Beechem,Gordon B. Mills +23 more
TL;DR: In this article , the authors demonstrate the ability of the CosMx platform to quantify more than 70 proteins encompassing key targets in immuno-oncology and tumor biology, localize the proteins and analyze protein expression at a single cell level.