Mylène Amherdt

TL;DR: In vitro synthesis of endoplasmic reticulum-derived transport vesicles has been reconstituted with washed membranes and three soluble proteins and it is proposed that the coat structures be called COPI and COPII.

TL;DR: Evidence is provided that suggests BFA's effects are not limited to the Golgi apparatus but are reiterated throughout the central vacuolar system.

TL;DR: Observations suggest that the assembly of the COPII coat on the ER occurs by a sequential binding of coat proteins to specific lipids and that this assembly promotes the budding of COPII-coated vesicles.

TL;DR: It is reported that brefeldin A prevents the assembly of non-clathrin-coated vesicles from Golgi cisternae in a cell-free system, and extensive tubule networks form that connect previously separate cisternAE and stacks into a single topological unit, allowing the intermixing of contents of Golgi bisternae by lateral diffusion.

TL;DR: Electron microscope immunocytochemistry reveals that both anterograde-directed and retrograde-directed cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicle budding from Golgi cisternae.