COPII-Coated Vesicle Formation Reconstituted with Purified Coat Proteins and Chemically Defined Liposomes
Ken Matsuoka,Lelio Orci,Mylène Amherdt,Sebastian Y. Bednarek,Susan Hamamoto,Randy Schekman,Thomas Yeung +6 more
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TLDR
Observations suggest that the assembly of the COPII coat on the ER occurs by a sequential binding of coat proteins to specific lipids and that this assembly promotes the budding of COPII-coated vesicles.About:
This article is published in Cell.The article was published on 1998-04-17 and is currently open access. It has received 629 citations till now. The article focuses on the topics: COPII & Coated vesicle.read more
Citations
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Journal ArticleDOI
The Mechanisms of Vesicle Budding and Fusion
TL;DR: Genetic and biochemical analyses of the secretory pathway have produced a detailed picture of the molecular mechanisms involved in selective cargo transport between organelles, including Vesicle budding and cargo selection, which depend on a machinery that includes the SNARE proteins.
Journal ArticleDOI
How proteins produce cellular membrane curvature
TL;DR: In this paper, the authors classify possible curvature-generating mechanisms that are provided by lipids that constitute the membrane bilayer and by proteins that interact with, or are embedded in, the membrane.
Journal ArticleDOI
Bi-directional protein transport between the ER and Golgi.
TL;DR: This work reviews the mechanisms that govern coat recruitment to the membrane, cargo capture into a transport vesicle, and accurate delivery to the target organelle.
Journal ArticleDOI
Protein-lipid interplay in fusion and fission of biological membranes.
TL;DR: The phenomenology and the pathways of the well-characterized reactions of biological remodeling, such as fusion mediated by influenza hemagglutinin, are compared with those studied for protein-free bilayers and some proteins involved in fusion and fission are considered.
Journal ArticleDOI
Functional partnership between amphiphysin and dynamin in clathrin-mediated endocytosis.
TL;DR: Results show that amphiphysin binds lipid bilayers, indicate a potential function for amphiphYSin in the changes in bilayer curvature that accompany vesicle budding, and imply a close functional partnership between amphiphisin and dynamin in endocytosis.
References
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COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum.
Charles Barlowe,Lelio Orci,Tom Yeung,Midori Hosobuchi,Susan Hamamoto,Nina R. Salama,M F Rexach,Mariella Ravazzola,Mylène Amherdt,Randy Schekman +9 more
TL;DR: In vitro synthesis of endoplasmic reticulum-derived transport vesicles has been reconstituted with washed membranes and three soluble proteins and it is proposed that the coat structures be called COPI and COPII.
Journal ArticleDOI
Protein Sorting by Transport Vesicles
TL;DR: Eukaryotic life depends on the spatial and temporal organization of cellular membrane systems and general principles that underlie a broad variety of physiological processes, including cell surface growth, the biogenesis of distinct intracellular organelles, endocytosis, and the controlled release of hormones and neurotransmitters.
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Coat Proteins and Vesicle Budding
Randy Schekman,Lelio Orci +1 more
TL;DR: The trafficking of proteins within eukaryotic cells is achieved by the capture of cargo and targeting molecules into vesicles that bud from a donor membrane and deliver their contents to a receiving compartment.
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Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting
Peter Schu,Kaoru Takegawa,Michael J. Fry,Jeffrey H. Stack,Michael D. Waterfield,Scott D. Emr +5 more
TL;DR: Overexpression of VPS34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p.
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Distinct sets of SEC genes govern transport vesicle formation and fusion early in the secretory pathway
Chris A. Kaiser,Randy Schekman +1 more
TL;DR: Mutations in two of the genes involved in vesicle fusion, SEC17 and SEC18, are lethal in combination, and five of six possible pairwise combinations of mutations in genes required for vesicles formation, SEC12, SEC13, SEC16, and SEC23, are fatal.
Related Papers (5)
Structure of the Sec23/24-Sar1 pre-budding complex of the COPII vesicle coat.
SEC12 encodes a guanine-nucleotide-exchange factor essential for transport vesicle budding from the ER
Charles Barlowe,Randy Schekman +1 more