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Nathan C. Shaner
Researcher at University of California, San Diego
Publications - 50
Citations - 12813
Nathan C. Shaner is an academic researcher from University of California, San Diego. The author has contributed to research in topics: Green fluorescent protein & mCherry. The author has an hindex of 23, co-authored 47 publications receiving 11705 citations. Previous affiliations of Nathan C. Shaner include RMIT University & Salk Institute for Biological Studies.
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Journal ArticleDOI
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Nathan C. Shaner,Robert E. Campbell,Robert E. Campbell,Paul Steinbach,Ben N G Giepmans,Amy E. Palmer,Roger Y. Tsien +6 more
TL;DR: The latest red version matures more completely, is more tolerant of N-terminal fusions and is over tenfold more photostable than mRFP1, and three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies.
Journal ArticleDOI
A guide to choosing fluorescent proteins.
TL;DR: A unified characterization of the best available FPs provides a useful guide in narrowing down the options for biological imaging tools.
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A bright monomeric green fluorescent protein derived from Branchiostoma lanceolatum.
Nathan C. Shaner,Gerard G. Lambert,Andrew Chammas,Yuhui Ni,Paula J. Cranfill,Michelle A. Baird,Brittney R. Sell,John R. Allen,Richard O. Day,Maria Israelsson,Michael W. Davidson,Jiwu Wang +11 more
TL;DR: In this article, a monomeric yellow green fluorescent protein, mNeonGreen, derived from a tetrameric fluorescent protein from the cephalochordate Branchiostoma lanceolatum, was described.
Journal ArticleDOI
Improving the photostability of bright monomeric orange and red fluorescent proteins.
Nathan C. Shaner,Michael Z. Lin,Michael R. McKeown,Paul Steinbach,Kristin L. Hazelwood,Michael W. Davidson,Roger Y. Tsien +6 more
TL;DR: This work developed highly photostable variants of mOrange and TagRFP that maintain most of the beneficial qualities of the original proteins and perform as reliably as Aequorea victoria GFP derivatives in fusion constructs.
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Advances in fluorescent protein technology
TL;DR: The relentless search for a bright, monomeric and fast-maturing red FP has yielded a host of excellent candidates, although none is yet optimal for all applications, and photoactivatable FPs are emerging as a powerful class of probes for intracellular dynamics and as useful tools for the development of superresolution microscopy applications.