N
Noelia Alonso
Researcher at Spanish National Research Council
Publications - 4
Citations - 370
Noelia Alonso is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Glutaraldehyde & Immobilized enzyme. The author has an hindex of 4, co-authored 4 publications receiving 350 citations.
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Journal ArticleDOI
Improved stabilization of chemically aminated enzymes via multipoint covalent attachment on glyoxyl supports
Fernando López-Gallego,Tamara Montes,Manuel Fuentes,Noelia Alonso,Valeria Grazú,Lorena Betancor,Jose M. Guisan,Roberto Fernandez-Lafuente +7 more
TL;DR: The surface carboxylic groups of penicillin G acylase and glutaryl acyl enzyme were chemically aminated in a controlled way by reaction with ethylenediamine via the 1-ethyl-3-(dimethylamino-propyl) carbodiimide coupling method and were immobilized on glyoxyl agarose.
Journal ArticleDOI
Co-aggregation of Enzymes and Polyethyleneimine: A Simple Method To Prepare Stable and Immobilized Derivatives of Glutaryl Acylase
Fernando López-Gallego,Lorena Betancor,Aurelio Hidalgo,Noelia Alonso,Roberto Fernandez-Lafuente,Jose M. Guisan +5 more
TL;DR: A new protocol for carrying out the preparation of cross-linked aggregates of enzymes with a low number of lysines on its surface is developed.
Journal ArticleDOI
Preparation of a robust biocatalyst of D-amino acid oxidase on sepabeads supports using the glutaraldehyde crosslinking method
Fernando López-Gallego,Lorena Betancor,Aurelio Hidalgo,Noelia Alonso,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +6 more
TL;DR: In this paper, different protocols to immobilize d -amino acid oxidase (DAAO) on sepabeads were assayed (ionic adsorption on different supports and covalent attachment using glutaraldehyde), studying the stability of the final preparations.
Journal ArticleDOI
Immobilization and stabilization of glutaryl acylase on aminated sepabeads supports by the glutaraldehyde crosslinking method
Noelia Alonso,Fernando López-Gallego,Lorena Betancor,Aurelio Hidalgo,Cesar Mateo,Jose M. Guisan,Roberto Fernandez-Lafuente +6 more
TL;DR: It was determined that immobilization on aminated supports increased the enzyme stability, and that this stabilization increased with the size of the polyethylenimine, which made this strategy very suitable for its industrial implementation.