Orsolya Kolacsek

TL;DR: It is demonstrated, for the first time, that the astrocyte network display synchronized recurrent activity in vivo coupled to UP states measured by field recording and neuronal calcium imaging, and conclusively suggest a causal role of theAstrocytic syncytium in SWA generation.

TL;DR: A transgene-independent method to determine copy numbers of transgenes delivered by the SB transposon system is developed, based on a quantitative real-time PCR detection method, offering a sensitive, non-radioactive, rapid and accurate approach, which has a potential to be used for gene therapy.

TL;DR: The parallel examination of in vivo cellular calcium dynamics and renal circulation by fluorescent probes opens new possibilities for physiologic and pharmacologic investigations.

TL;DR: A human embryonic stem cell line stably expressing a genetically encoded Ca(2+) indicator (GCaMP2) using a transposon-based gene delivery system is developed and it is found that the differentiation properties were fully preserved in the GCa MP2-expressing hESC lines and Ca imaging could be performed without the need of toxic dye-loading of the cells.

TL;DR: It is concluded that high excision activity is not always followed by a higher transgenic rate, as exemplified by the hyperactive transposases, indicating that the excision and the integration steps of transposition are not strongly coupled as previously thought.