P. Dierks

TL;DR: Cloned rabbit beta-globin genes, modified in vitro by restructuring or site-directed mutagenesis, were introduced into mouse 3T6 cells, and the resulting transcripts were analyzed by nuclease S1 mapping to find the first 109 bp preceding the cap site sufficed for maximal beta- globin transcription.

TL;DR: Mouse thymidine kinase-negative (TK-) L cells were transformed with concatenates of cloned herpes simplex virus 1 TK DNA and different rabbit beta-globin DNAs in which the globin genes were preceded by native flanking sequences of 14, 66, 76, 425, and 1500 nucleotides.

TL;DR: A hybrid gene consisting of the human IFN-alpha 1 promoter and a beta-globin transcription unit is expressed correctly only after viral induction, and induction is attributed largely to positive, rather than to negative control.

TL;DR: There was a striking difference in the proportion of individual IFN-alpha mRNA species in different cell types, in particular between normal and leukaemic cells; for example all cases of myeloblastic leukaemia examined showed a high expression of IFn-alpha 14.

TL;DR: In this article, the effect of modifications introduced into the 5′-flanking region of the rabbit β-globin gene on correct transcription was determined in vivo and in vitro, and a region 57 to 84 bp upstream of the cap site, containing the CAAT box, had a potentiating effect on the level of transcription in vivo but was not required for correct initiation.