Dipolar coupling interactions represent an extremely valuable source of long-range distance and angle information that was previously not available for solution structure determinations of macromolecules. This is because observation of these dipolar coupling data requires creating an anisotropic environment for the macromolecule. Here we introduce a new method for generating tunable degrees of alignment of macromolecules by addition of magnetically aligned Pf1 filamentous bacteriophage as a cosolute. This phage-induced alignment technique has been used to study 1H-1H, 1H-13C, and 1H-15N dipolar coupling interactions in a DNA duplex, an RNA hairpin and several proteins including thioredoxin and apo-calmodulin. The phage allow alignment of macromolecules over a wide range of temperature and solution conditions and thus represent a stable versatile method for generating partially aligned macromolecules in solution.

Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions

Carbohydrate structural determination by NMR spectroscopy: modern methods and limitations

http://www.cs.duke.edu/brd/Teaching/Bio/asmb/current/Papers/RDC.SVD.Prestegard.pdf

Order matrix analysis of residual dipolar couplings using singular value decomposition.

Dipolar couplings in macromolecular structure determination.

2. Theoretical treatment of dipolar interactions 376 2.1 Anisotropic interactions as probes of macromolecular structure and dynamics 376 2.1.1 The dipolar interaction 376 2.1.2 Averaging in the solution state 377 2.2 Ordering of a rigid body 377 2.2.1 The Saupe order tensor 378 2.2.2 Orientational probability distribution function 380 2.2.3 The generalized degree of order 380 2.3 Molecular structure and internal dynamics 381

https://www.cambridge.org/core/services/aop-cambridge-core/content/view/S0033583500003656

NMR structures of biomolecules using field oriented media and residual dipolar couplings.

Molecular symmetry as an aid to geometry determination in ligand protein complexes.

Anti-human immunodeficiency and anti-hepatitis B virus activities of β-l-2′,3′-dideoxy purine nucleosides

Intensity-Based Measurement of Homonuclear Residual Dipolar Couplings from CT-COSY

Cosy cross-peaks from 1h-1h dipolar couplings in nmr spectra of field oriented oligosaccharides

A general method for the synthesis of 2′-C-α-methyl-2′,3′-dideoxynucleosides is presented. Stereofacial selectivity of the 2-C-methylation reaction of γ-lactone has been investigated, in which the presence of a bulky group at the 5-hydroxymethyl produced the α-isomer as a major product. During glycosylation, the α-methyl group directed the formation of nucleosides in favor of the s-isomer. This methodology is applied to the synthesis of some new pyrimidine and purine nucleosides. This manuscript is dedicated to the celebration of Prof. Yoshihisa Mizuno's 75th birthday.

P. J. Bolon

Papers

Molecular symmetry as an aid to geometry determination in ligand protein complexes.

Anti-human immunodeficiency and anti-hepatitis B virus activities of β-l-2′,3′-dideoxy purine nucleosides

Intensity-Based Measurement of Homonuclear Residual Dipolar Couplings from CT-COSY

Cosy cross-peaks from 1h-1h dipolar couplings in nmr spectra of field oriented oligosaccharides

Synthesis of 2′-C-α-Methyl-2′,3′-dideoxynucleosides