P
Pablo E. Visconti
Researcher at University of Massachusetts Amherst
Publications - 142
Citations - 12403
Pablo E. Visconti is an academic researcher from University of Massachusetts Amherst. The author has contributed to research in topics: Capacitation & Sperm. The author has an hindex of 55, co-authored 135 publications receiving 11271 citations. Previous affiliations of Pablo E. Visconti include University of Pennsylvania & University of Virginia.
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Journal ArticleDOI
Capacitation of mouse spermatozoa. I. Correlation between the capacitation state and protein tyrosine phosphorylation.
Pablo E. Visconti,Janice L. Bailey,Grace D. Moore,Dieyun Pan,Patricia Olds-Clarke,Gregory S. Kopf +5 more
TL;DR: Caput epididymal sperm, which lack the ability to undergo capacitation in vitro, do not display this capacitation-dependent subset of tyrosine phosphorylated proteins in complete media even after extended incubation periods, and do not fertilize metaphase II-arrested eggs in vitro.
Journal ArticleDOI
Capacitation of mouse spermatozoa. II. Protein tyrosine phosphorylation and capacitation are regulated by a cAMP-dependent pathway.
Pablo E. Visconti,Grace D. Moore,Janice L. Bailey,P. Leclerc,Stephanie A. Connors,Dieyun Pan,Patricia Olds-Clarke,Gregory S. Kopf +7 more
TL;DR: Up-regulation of protein tyrosine phosphorylation by cAMP/PKA in sperm is, to the authors' knowledge, the first demonstration of such an interrelationship between tyrosin kinase/phosphatase and PKA signaling pathways.
Journal ArticleDOI
Regulation of protein phosphorylation during sperm capacitation
TL;DR: Capacitation in vitro has been accomplished using cauda and/or ejaculated sperm incubated under a variety of conditions in defined media that mimic the electrolyte composition of the oviduct fluid, and the action of these media components to promote capacitation at the molecular level is poorly understood.
Journal ArticleDOI
Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation.
Scott B. Ficarro,Olga Chertihin,V. Anne Westbrook,Forest M. White,Friederike L. Jayes,Petr Kalab,Jarrod A. Marto,Jeffrey Shabanowitz,John C. Herr,Donald F. Hunt,Pablo E. Visconti +10 more
TL;DR: The phosphopeptide enrichment and quantification methodology coupled to MS/MS, described here for the first time, can be employed to map and compare phosphorylation sites involved in multiple cellular processes.
Journal ArticleDOI
Regulation of Protein Tyrosine Phosphorylation during Bovine Sperm Capacitation by a Cyclic Adenosine 3',5'-Monophosphate-Dependent Pathway
TL;DR: These studies are the first to report a unique interrelationship between tyrosine kinase/phosphatase and cAMP signaling pathways at the level of PK-A in bovine sperm capacitation.