P
Petr Kalab
Researcher at Johns Hopkins University
Publications - 55
Citations - 4308
Petr Kalab is an academic researcher from Johns Hopkins University. The author has contributed to research in topics: Ran & Nuclear pore. The author has an hindex of 26, co-authored 52 publications receiving 3941 citations. Previous affiliations of Petr Kalab include University of Pennsylvania & Czechoslovak Academy of Sciences.
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Journal ArticleDOI
Visualization of a Ran-GTP Gradient in Interphase and Mitotic Xenopus Egg Extracts
TL;DR: During interphase, Ran-GTP was highly enriched in the nucleoplasm, and a steep concentration difference between nuclear and cytoplasmic Ran-gTP was established, providing evidence for a Ran- GTP gradient surrounding chromosomes throughout the cell cycle.
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Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein/p97 during capacitation.
Scott B. Ficarro,Olga Chertihin,V. Anne Westbrook,Forest M. White,Friederike L. Jayes,Petr Kalab,Jarrod A. Marto,Jeffrey Shabanowitz,John C. Herr,Donald F. Hunt,Pablo E. Visconti +10 more
TL;DR: The phosphopeptide enrichment and quantification methodology coupled to MS/MS, described here for the first time, can be employed to map and compare phosphorylation sites involved in multiple cellular processes.
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Analysis of a RanGTP-regulated gradient in mitotic somatic cells
TL;DR: The results demonstrate that conserved Ran-regulated pathways are involved in multiple, parallel processes required for spindle function, but that their relative contribution differs in chromatin- versus centrosome/kinetochore-driven spindle assembly systems.
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The ran GTPase regulates mitotic spindle assembly.
TL;DR: Evidence is presented that the role of Ran in spindle assembly is independent of nuclear transport and is probably mediated through changes in microtubule dynamics, as well as how increased concentrations of RanBP1 disturb mitosis.
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The RanGTP gradient – a GPS for the mitotic spindle
Petr Kalab,Rebecca Heald +1 more
TL;DR: The RanGTP gradient is conserved, although its roles vary among different cell types and species, and much remains to be learned regarding its functions.