Patrick Courtney

TL;DR: The nature of the Ser/Thr/Tyr phosphoproteome in Bacillus subtilis is established using in vivo labelling with [32P]‐orthophosphate, one‐unit pH 2‐DE, combined with MS, and significant changes to the profiles were obtained as a result of cold, heat or osmotic shock, demonstrating that, in stationary‐phase cells, the phosphoproteinome is dynamic.

TL;DR: The application of this time domain optically sectioned fluorescence lifetime imaging microscope to real-time monitoring of changes in lipid order in cell membranes following cholesterol depletion using cyclodextrin and to the activation of the small GTP-ase Ras in live cells using FRET is demonstrated.

TL;DR: What is seen as current best practices in algorithmic novelty and the increasing importance of validation on particular data sets and problems are reviewed and refinements that may benefit the field of computer vision are suggested.

TL;DR: In this paper, a method for the detection of light emanating from fluorescent species present in a sample in order to study the structure and dynamics of such a sample is presented, which includes irradiating the sample with a pulse of excitation energy causing fluorescent species in the sample to fluoresce; detecting light emanating emanating from the sample during a predetermined period of time after the pulse; generating and storing data recording at least the wavelength of the detected light against time; and analysing the data with reference to the respective lifetimes of the fluorescent species to detect the presence of the respective emissions

TL;DR: An optically sectioned fluorescence lifetime imaging microscope with a wide-field detector is demonstrated, using a convenient, continuously tunable (435-1150 nm) ultrafast source for fluorescence imaging applications that is derived from a visible supercontinuum generated in a microstructured fiber.