P
Paul Steinbach
Researcher at University of California, San Diego
Publications - 20
Citations - 13732
Paul Steinbach is an academic researcher from University of California, San Diego. The author has contributed to research in topics: Cancer & Green fluorescent protein. The author has an hindex of 14, co-authored 20 publications receiving 12920 citations. Previous affiliations of Paul Steinbach include Howard Hughes Medical Institute.
Papers
More filters
Journal ArticleDOI
Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein.
Nathan C. Shaner,Robert E. Campbell,Robert E. Campbell,Paul Steinbach,Ben N G Giepmans,Amy E. Palmer,Roger Y. Tsien +6 more
TL;DR: The latest red version matures more completely, is more tolerant of N-terminal fusions and is over tenfold more photostable than mRFP1, and three monomers with distinguishable hues from yellow-orange to red-orange have higher quantum efficiencies.
Journal ArticleDOI
A guide to choosing fluorescent proteins.
TL;DR: A unified characterization of the best available FPs provides a useful guide in narrowing down the options for biological imaging tools.
Journal ArticleDOI
A monomeric red fluorescent protein
Robert E. Campbell,Oded Tour,Amy E. Palmer,Paul Steinbach,Geoffrey S. Baird,David A. Zacharias,Roger Y. Tsien +6 more
TL;DR: This work presents the stepwise evolution of DsRed to a dimer and then either to a genetic fusion of two copies of the protein, i.e., a tandem dimer, or to a true monomer designated mRFP1 (monomeric red fluorescent protein).
Journal ArticleDOI
Improving the photostability of bright monomeric orange and red fluorescent proteins.
Nathan C. Shaner,Michael Z. Lin,Michael R. McKeown,Paul Steinbach,Kristin L. Hazelwood,Michael W. Davidson,Roger Y. Tsien +6 more
TL;DR: This work developed highly photostable variants of mOrange and TagRFP that maintain most of the beneficial qualities of the original proteins and perform as reliably as Aequorea victoria GFP derivatives in fusion constructs.
Journal ArticleDOI
Characterization of Engineered Channelrhodopsin Variants with Improved Properties and Kinetics
TL;DR: Point mutation of Ile(170) of ChEF to Val (yielding "ChIEF") accelerates the rate of channel closure while retaining reduced inactivation, leading to more consistent responses when stimulated above 25 Hz in both HEK293 cells and cultured hippocampal neurons.