Pavol Miskovsky

TL;DR: The present results confirm the high sensitivity of SERS for the detection of the organochlorine pesticides with a limit of detection reaching 10(-8) M, thus providing a solid basis for the construction of suitable nanosensors for the identification and quantitative analysis of this type of chemical.

TL;DR: Confocal laser microspectrofluorometric measurements on human T47D mammary tumor cells show that, while being primarily located in the cell membrane and cytoplasm after a short‐term incubation in a 10−6M hypericin‐containing culture medium,hypericin actually reaches the inside of the cell nucleus after a long‐ term incubation.

TL;DR: It is demonstrated that the drug/protein interaction can take place through two different binding sites which are probably localized in the IIA and IIIA hydrophobic pockets of HSA and which correspond to Sudlow's I and II binding sites, respectively.

TL;DR: The colocalization experiments showed the lysosomal localization of Hyp following the uptake and the concentration of Hyp in these organelles was enhanced in the cells with elevated number of LDL receptors when the incubation medium contained LDL, suggesting that LDL and LDL receptor‐pathway play an important role in the delivery and accumulation of Hyp into the cells.

TL;DR: It is shown, that the observed photo-induced cell deaths can be correlated with the sub-cellular distribution of the active fluorescent monomer form of Hyp in lysosomes, but that possible aggregation in some organelles should be taken into account for interpretation of the real dynamics of the subcellular redistribution.