P
Perng-Kuang Chang
Researcher at United States Department of Agriculture
Publications - 73
Citations - 3964
Perng-Kuang Chang is an academic researcher from United States Department of Agriculture. The author has contributed to research in topics: Aspergillus flavus & Aspergillus parasiticus. The author has an hindex of 36, co-authored 67 publications receiving 3632 citations. Previous affiliations of Perng-Kuang Chang include Tulane University & Michigan State University.
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Journal ArticleDOI
Comparative mapping of aflatoxin pathway gene clusters in Aspergillus parasiticus and Aspergillus flavus.
Jiujiang Yu,Perng-Kuang Chang,Jeffrey W. Cary,Maureen S. Wright,Deepak Bhatnagar,Thomas E. Cleveland,Gary A. Payne,John E. Linz +7 more
TL;DR: The physical distances (in kilobase pairs) and the directions of transcription of these genes have been determined for both aflatoxigenic species and the order is related to the order in enzymatic steps required for aflatoxin biosynthesis.
Journal ArticleDOI
Sequence breakpoints in the aflatoxin biosynthesis gene cluster and flanking regions in nonaflatoxigenic Aspergillus flavus isolates.
TL;DR: Genetic drift may be a driving force that is responsible for the loss of the entire aflatoxin gene cluster in nonaflatoxigenic A. flavus isolates when aflatoxins have lost their adaptive value in nature.
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Increased expression of Aspergillus parasiticus aflR, encoding a sequence-specific DNA-binding protein, relieves nitrate inhibition of aflatoxin biosynthesis.
TL;DR: Results suggest that aflR is involved in the regulation of multiple parts of the aflatoxin biosynthetic pathway.
Journal ArticleDOI
Cloning of the Aspergillus parasiticus apa-2 gene associated with the regulation of aflatoxin biosynthesis.
Perng-Kuang Chang,Jeffrey W. Cary,Deepak Bhatnagar,T. E. Cleveland,Joan W. Bennett,John E. Linz,C. P. Woloshuk,Gary A. Payne +7 more
TL;DR: An Aspergillus parasiticus gene, designated apa-2, was identified as a regulatory gene associated with aflatoxin biosynthesis and was found to complement an A. flavus afl-2 mutant strain for a flatoxin production, suggesting that apA-2 is functionally homologous to aFL-2.
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Isolation and characterization of a gene from Aspergillus parasiticus associated with the conversion of versicolorin A to sterigmatocystin in aflatoxin biosynthesis.
TL;DR: DNA isolated from the wild-type aflatoxin-producing fungus Aspergillus parasiticus NRRL 5862 was used to construct a cosmid genomic DNA library employing the homologous gene (pyrG) encoding orotidine monophosphate decarboxylase for selection of fungal transformants, revealing striking similarity with Streptomyces ketoreductases involved in polyketide biosynthesis.