Peter Chovanec

TL;DR: Complete profiling of cell surface proteins by flow cytometry in naive and primed human PSCs revealed distinct transcriptional and X chromosome inactivation changes associated with the early and late stages of naive cell formation.

TL;DR: In this paper, the spatial organization of RNA and DNA in the nucleus has been comprehensively mapped to reveal higher-order RNA-chromatin structures associated with three major classes of nuclear function: RNA processing, heterochromatin assembly, and gene regulation.

TL;DR: This work developed RNA & DNA SPRITE (RD-SPRITE) to comprehensively map the location of all RNAs relative to DNA and other RNAs and demonstrates a unique mechanism by which RNA acts to shape nuclear structure by forming high concentration territories immediately upon transcription, binding to diffusible regulators, and guiding them into spatial compartments to regulate a wide range of essential nuclear functions.

TL;DR: Local chromatin signatures downstream of VH genes provide an essential layer of regulation that determines recombination efficiency, and VDJ sequencing (VDJ-seq), a DNA-based next-generation-sequencing technique that quantitatively profiles recombination products, is developed.

TL;DR: Adapting VDJ-seq to profile the Igκ VκJκ repertoire and presenting a comprehensive readout in mouse pre-B cells reveals highly variable Vκ gene usage and provides avenues for further investigation of chromatin signatures that may underpin V(D)J-mediated chromosomal translocations.