Peter J. Cox

TL;DR: The role of acrolein as the causative agent in cyclophosphamide cystitis was proven and N-acetyl-l-cysteine protection against this toxicity was demonstrated.

TL;DR: It appeared that, of the known metabolites of cyclophosphamide, only phosphoramide mustard possesses the cytoxicity and biological half-life appropriate to the active antitumour metabolite, and four other metabolites of low cytotoxicity were isolated and identified.

TL;DR: 4-Methylcycloph phosphamide cannot form metabolites analogous to 4-ketocyclophosphamide and carboxyphosphamide, the relatively non-toxic metabolites of cyclophosphamia, and the significance of this fact is discussed in relation to a mechanism which could account for the relatively selective cytotoxicity of cyclophile in vivo towards neoplastic tissue.

TL;DR: The rate of metabolism and the antitumour activity of cyclophosphamide and its 4-d2 analogue were compared and the mass spectrum of the deuterated acrolein 2,4-dinitrophenylhydrazone obtained from the total reactive metabolites was used to estimate the ratio of 4- to 6-hydroxylation.

TL;DR: The major product isolated from the oxidation of the carcinogen, N-nitrosopiperidine, by the ascorbic acid model system of Udenfriend et al. has been identified as N-Nitroso-4-piperidone both by its spectral properties and by comparison with authentic material.