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Philipp D. Pollheimer
Researcher at Johannes Kepler University of Linz
Publications - 9
Citations - 536
Philipp D. Pollheimer is an academic researcher from Johannes Kepler University of Linz. The author has contributed to research in topics: Receptor & Avidin. The author has an hindex of 8, co-authored 9 publications receiving 499 citations. Previous affiliations of Philipp D. Pollheimer include Hong Kong University of Science and Technology & Medical University of Vienna.
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Journal ArticleDOI
Linking of Sensor Molecules with Amino Groups to Amino-Functionalized AFM Tips
Linda Wildling,Barbara Unterauer,Rong Zhu,Anne Rupprecht,Thomas Haselgrübler,Christian Rankl,Andreas Ebner,Doris Vater,Philipp D. Pollheimer,Elena E. Pohl,Peter Hinterdorfer,Hermann J. Gruber +11 more
TL;DR: A uncompromising solution to the problem of undesired bivalent reaction of bifunctional linkers with adjacent NH2 groups on the tip surface was found with the help of a new cross-linker (“acetal-PEG-NHS”) which possesses one activated carboxyl group and one acetal-protected benzaldehyde function.
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Single-molecule force spectroscopy and imaging of the vancomycin/D-Ala-D-Ala interaction.
Yann Gilbert,Marie Deghorain,Ling Wang,Binghe B. Xu,Philipp D. Pollheimer,Hermann J Gruber,Jeff Errington,Bernard Hallet,Xavier Haulot,Claire Verbelen,Pascal Hols,Yves F. Dufrêne +11 more
TL;DR: The ability of single-molecule atomic force microscopy with antibiotic-modified tips to measure the specific binding forces of vancomycin and to map individual ligands on living bacteria is demonstrated.
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Antibody linking to atomic force microscope tips via disulfide bond formation.
A. S. M. Kamruzzahan,Andreas Ebner,Linda Wildling,Ferry Kienberger,Christian K. Riener,Christoph D. Hahn,Philipp D. Pollheimer,Peter Winklehner,Martin Hölzl,Bernd Lackner,Daniela M Schörkl,Peter Hinterdorfer,Hermann J. Gruber +12 more
TL;DR: The coupling scheme was well-reproduced on amino-functionalized silicon nitride chips, and the number of covalently bound biotin-IgG per microm2 was estimated by the amount of specifically bound ExtrAvidin-peroxidase conjugate.
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Molecular Determinants within N Terminus of Orai3 Protein That Control Channel Activation and Gating
Judith Bergsmann,Isabella Derler,Martin Muik,Irene Frischauf,Marc Fahrner,Philipp D. Pollheimer,Clemens Schwarzinger,Hermann J. Gruber,Klaus Groschner,Christoph Romanin +9 more
TL;DR: In aggregate, the N-terminal conserved region plays a multifaceted role in Orai3 current gating with distinct structural requirements for STIM1- and 2-APB-stimulated activation.
Journal ArticleDOI
Nanoscale DNA Tetrahedra Improve Biomolecular Recognition on Patterned Surfaces
Robert Schlapak,Jürgen Danzberger,David Armitage,David J. Morgan,Andreas Ebner,Peter Hinterdorfer,Philipp D. Pollheimer,Hermann J. Gruber,Friedrich Schäffler,Stefan Howorka +9 more
TL;DR: In assays testing the molecular accessibility via DNA hybridization and protein capturing, tetrahedron-tethered receptors outperformed conventional immobilization approaches with regard to specificity and amount of captured polypeptide by a factor of up to seven.