Linking of Sensor Molecules with Amino Groups to Amino-Functionalized AFM Tips
Linda Wildling,Barbara Unterauer,Rong Zhu,Anne Rupprecht,Thomas Haselgrübler,Christian Rankl,Andreas Ebner,Doris Vater,Philipp D. Pollheimer,Elena E. Pohl,Peter Hinterdorfer,Hermann J. Gruber +11 more
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TLDR
A uncompromising solution to the problem of undesired bivalent reaction of bifunctional linkers with adjacent NH2 groups on the tip surface was found with the help of a new cross-linker (“acetal-PEG-NHS”) which possesses one activated carboxyl group and one acetal-protected benzaldehyde function.About:
This article is published in Bioconjugate Chemistry.The article was published on 2011-06-15 and is currently open access. It has received 155 citations till now. The article focuses on the topics: Bifunctional.read more
Citations
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Molecular interaction and inhibition of SARS-CoV-2 binding to the ACE2 receptor.
Jinsung Yang,Simon J. L. Petitjean,Melanie Koehler,Qingrong Zhang,Andra C. Dumitru,Wenzhang Chen,Sylvie Derclaye,Stéphane P. Vincent,Patrice Soumillion,David Alsteens +9 more
TL;DR: It is demonstrated, both on model surfaces and on living cells, that the receptor binding domain (RBD) serves as the binding interface within the S-glycoprotein with the ACE2 receptor and the kinetic and thermodynamic properties of this binding pocket are extracted.
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Nanomechanical mapping of soft materials with the atomic force microscope: methods, theory and applications.
TL;DR: This review introduces the state-of-the-art force microscope-based methods to map at high-spatial resolution the elastic and viscoelastic properties of soft materials.
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Nanomechanical mapping of first binding steps of a virus to animal cells.
David Alsteens,Richard Newton,Rajib Schubert,David Martinez-Martin,Martin Delguste,Botond Roska,Daniel J. Müller +6 more
TL;DR: An atomic force and confocal microscopy set-up is shown that allows the surface receptor landscape of cells to be imaged and the virus binding events within the first millisecond of contact with the cell to be mapped at high resolution.
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Imaging G protein–coupled receptors while quantifying their ligand-binding free-energy landscape
David Alsteens,Moritz Pfreundschuh,Cheng Zhang,Patrizia M. Spoerri,Shaun R. Coughlin,Brian K. Kobilka,Daniel J. Müller +6 more
TL;DR: This work uses force-distance curve–based atomic force microscopy to simultaneously image single native G protein–coupled receptors in membranes and quantify their dynamic binding strength to native and synthetic ligands.
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Peptide-mediated desmoglein 3 crosslinking prevents pemphigus vulgaris autoantibody-induced skin blistering
Volker Spindler,Vera Rötzer,Carina Dehner,Bettina Kempf,Martin Gliem,Mariya Y. Radeva,Eva Hartlieb,Gregory S. Harms,Enno Schmidt,Jens Waschke +9 more
TL;DR: TP data indicate that p38 MAPK links autoantibody-mediated inhibition of DSG3 binding to skin blistering, and may serve as a promising treatment option for pemphigus vulgaris.
References
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Calibration of atomic‐force microscope tips
TL;DR: In this article, the authors describe a simple, nondestructive procedure for measuring the force constant, resonant frequency, and quality factor of an AFM cantilever spring and the effective radius of curvature of an AU tip.
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Dynamic strength of molecular adhesion bonds.
Evan Evans,Ken Ritchie +1 more
TL;DR: How Brownian dynamics can help bridge the gap between molecular dynamics and probe tests is described, which shows that bond strength progresses through three dynamic regimes of loading rate.
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Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.
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Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors
TL;DR: The biospecific interaction of the immobilized ligand with its biological counterpart is illustrated by the effects on the interaction of immunoglobulins with immobilized Staphylococcus aureus protein A for various amounts of protein A.
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Detection and localization of individual antibody-antigen recognition events by atomic force microscopy
TL;DR: It is indicated that this methodology has promise for characterizing rate constants and kinetics of molecular recognition complexes and for molecular mapping of biosurfaces such as membranes.