P
Philippe Vain
Researcher at Norwich Research Park
Publications - 24
Citations - 1500
Philippe Vain is an academic researcher from Norwich Research Park. The author has contributed to research in topics: Brachypodium distachyon & Brachypodium. The author has an hindex of 17, co-authored 24 publications receiving 1425 citations. Previous affiliations of Philippe Vain include Norwich University.
Papers
More filters
Journal ArticleDOI
Molecular characterization of transforming plasmid rearrangements in transgenic rice reveals a recombination hotspot in the CaMV 35S promoter and confirms the predominance of microhomology mediated recombination.
Ajay Kohli,Simon Griffiths,Natalia Palacios,Richard M. Twyman,Philippe Vain,David A. Laurie,Paul Christou +6 more
TL;DR: Clustering of plasmid recombination events in this highly recombinogenic region, even in the absence of viral enzymes and other cis-acting elements proves that the plant cellular machinery alone is sufficient to recognize and act on these viral sequences.
Journal ArticleDOI
Brachypodium distachyon: making hay with a wild grass
TL;DR: Brachypodium distachyon is a wild grass with a short life cycle and accessions collected from across its ancestral range show a surprising degree of phenotypic variation in many traits, including those implicated in domestication of the cereals.
Journal ArticleDOI
Transgene expression in rice engineered through particle bombardment: molecular factors controlling stable expression and transgene silencing
TL;DR: It is observed that the presence of truncated sequences of transgenes capable of generating incomplete transcripts, resulting in aberrant RNA species, may be responsible for silencing in transgenic rice lines generated through particle-bombardment-mediated transformation.
Journal ArticleDOI
A protocol for Agrobacterium-mediated transformation of Brachypodium distachyon community standard line Bd21.
TL;DR: An improved procedure is provided for the facile and efficient Agrobacterium-mediated transformation of line Bd21, a novel model system for structural and functional genomics studies of temperate grasses because of its biological and genetic attributes.
Journal ArticleDOI
Agrobacterium-mediated transformation of the temperate grass Brachypodium distachyon (genotype Bd21) for T-DNA insertional mutagenesis
Philippe Vain,Barbara Worland,Vera Thole,Neil McKenzie,Sílvia C Alves,Magdalena Opanowicz,Lesley Fish,Michael W. Bevan,John W. Snape +8 more
TL;DR: A facile, efficient and rapid transformation system for Bd21 is described using Agrobacterium-mediated transformation of compact embryogenic calli derived from immature embryos to facilitate large-scale functional genomics research in this model system.