Pierre Sabatier

TL;DR: Compared to standard approaches where curves are fitted to protein solubility data acquired at different temperatures and drug concentrations, Proteome Integral Solubility Alteration (PISA) assay increases the analysis throughput by one to two orders of magnitude for unlimited number of factor variation points in such a scheme.

TL;DR: The orthogonality and complementarity of different chemical proteomics methods that can furnish invaluable mechanistic information and thus the approach can facilitate drug discovery efforts in general are assessed.

TL;DR: In this article, a thermal shift-based method called System-Wide Identification and prioritization of Enzyme Substrates by Thermal Analysis (SIESTA) was proposed to identify enzyme substrates from cell lysates.

TL;DR: A proteome signature library of drug-treated cancer cell lines is generated and a software tool to deconvolute drug targets and gain insights into their mechanisms of action is developed.

TL;DR: Adding detached cells to the expression proteomics analysis of drug-treated cells can significantly increase the analytical value of the approach, and provide further insight into cell life and death decision making.