Quentin Gouil

TL;DR: The current landscape of available tools is reviewed, the principles of error correction, base modification detection, and long-read transcriptomics analysis are focused on, and the challenges that remain are highlighted.

TL;DR: Analysis of DNA methylation patterns in trinucleotide contexts in four angiosperm species reveals that the sequence context of the methylome of plant genomes is informative about the mechanisms associated with maintenance of methylation and the overlying chromatin structure.

TL;DR: This study reveals the complex dynamics and interactions of the transcriptome and epigenome during seed germination, including the extensive remodelling of the seed DNA methylome from an embryo-like to vegetative-like state during the seed-to-seedling transition.

TL;DR: Using long-read nanopore sequencing it is shown that, with an average genomic coverage of ∼10, it is possible to determine both the level of methylation of CpG sites and the haplotype from which each read arises, and successfully identifies known imprinting control regions (ICRs) as well as some novel differentially methylated regions which may represent new ICRs.

TL;DR: The ability to detect various modified bases from a universal sample preparation, in addition to the mapping and phasing advantages of the longer read lengths, provide long-read sequencing with a decisive edge over short-read bisulfite sequencing for an expanding number of applications across kingdoms.