R
Rafael Núñez-Ramírez
Researcher at Spanish National Research Council
Publications - 27
Citations - 1379
Rafael Núñez-Ramírez is an academic researcher from Spanish National Research Council. The author has contributed to research in topics: Helicase & DNA. The author has an hindex of 15, co-authored 26 publications receiving 1193 citations.
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Journal ArticleDOI
Image processing for electron microscopy single-particle analysis using XMIPP
Sjors H.W. Scheres,Rafael Núñez-Ramírez,Carlos Oscar S. Sorzano,José María Carazo,Roberto Marabini +4 more
TL;DR: A collection of standardized image processing protocols for electron microscopy single-particle analysis using the XMIPP software package that allows performing the entire processing workflow starting from digitized micrographs up to the final refinement and evaluation of 3D models.
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The Antibacterial Cell Division Inhibitor PC190723 Is an FtsZ Polymer-stabilizing Agent That Induces Filament Assembly and Condensation
José Manuel Andreu,Claudia Schaffner-Barbero,Sonia Huecas,Dulce Alonso,María L. López-Rodríguez,Laura B. Ruiz-Avila,Rafael Núñez-Ramírez,Oscar Llorca,Antonio J. Martin-Galiano +8 more
TL;DR: The biochemical action of PC190723 on FtsZ parallels that of the microtubule-stabilizing agent taxol on the eukaryotic structural homologue tubulin, and it is yet to be investigated whether both ligands target structurally related assembly switches.
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3D architecture of DNA Pol α reveals the functional core of multi‐subunit replicative polymerases
TL;DR: X‐ray crystallography and electron microscopy are combined to describe subunit interaction and 3D architecture of heterodimeric yeast Pol α and provide a structural template for the common functional architecture of the three major replicative DNA polymerases.
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Iterative elastic 3D-to-2D alignment method using normal modes for studying structural dynamics of large macromolecular complexes
Qiyu Jin,Carlos Oscar S. Sorzano,José Miguel de la Rosa-Trevín,J.R. Bilbao-Castro,Rafael Núñez-Ramírez,Oscar Llorca,Florence Tama,Slavica Jonic +7 more
TL;DR: HEMNMA was tested with synthetic and experimental data sets of E. coli 70S ribosome, DNA polymerase Pol α and B subunit complex of the eukaryotic primosomes, and tomato bushy stunt virus and may be used in combination with classic discrete procedures.
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The nonsense-mediated mRNA decay SMG-1 kinase is regulated by large-scale conformational changes controlled by SMG-8
Ernesto Arias-Palomo,Akio Yamashita,Israel S. Fernández,Israel S. Fernández,Rafael Núñez-Ramírez,Yumi Bamba,Natsuko Izumi,Shigeo Ohno,Oscar Llorca +8 more
TL;DR: Large-scale conformational changes induced by SMG-8 afterSMG-9-mediated recruitment tune SMG -1 kinase activity to modulate NMD.