After having reviewed some aspects of microfluidic system preparation in the first part (1), in this second part of the review we will cover a number of standard operations (namely: sample preparation, sample injection, sample manipulation, reaction, separation, and detection) as well as some biological applications of micro total analysis systems (namely: cell culture, polymerase chain reaction, DNA separation, DNA sequencing, and clinical diagnostics). As previously, we will include papers issued from different scientific journals as well as useful abstracts from three conference proceedings: MEMS, Transducers, and μTAS. In this second part, we do not include the period covered by the history section (1975-1997) from part 1 but try to cover the relevant examples of the literature published between January 1998 and March 2002. We briefly describe articles that struck us as needing special attention, while more “standard” papers are dutifully reported in groups of interest. An article might be included in more than one section, depending on the ideas developed in it.

Micro total analysis systems. 2. Analytical standard operations and applications.

A self-scanned 1024 element photodiode array and minicomputer are used to measure the phase (wavefront) in the interference pattern of an interferometer to lambda/100. The photodiode array samples intensities over a 32 x 32 matrix in the interference pattern as the length of the reference arm is varied piezoelectrically. Using these data the minicomputer synchronously detects the phase at each of the 1024 points by a Fourier series method and displays the wavefront in contour and perspective plot on a storage oscilloscope in less than 1 min (Bruning et al. Paper WE16, OSA Annual Meeting, Oct. 1972). The array of intensities is sampled and averaged many times in a random fashion so that the effects of air turbulence, vibrations, and thermal drifts are minimized. Very significant is the fact that wavefront errors in the interferometer are easily determined and may be automatically subtracted from current or subsequent wavefrots. Various programs supporting the measurement system include software for determining the aperture boundary, sum and difference of wavefronts, removal or insertion of tilt and focus errors, and routines for spatial manipulation of wavefronts. FFT programs transform wavefront data into point spread function and modulus and phase of the optical transfer function of lenses. Display programs plot these functions in contour and perspective. The system has been designed to optimize the collection of data to give higher than usual accuracy in measuring the individual elements and final performance of assembled diffraction limited optical systems, and furthermore, the short loop time of a few minutes makes the system an attractive alternative to constraints imposed by test glasses in the optical shop.

Digital wavefront measuring interferometer for testing optical surfaces and lenses

Micro total analysis systems. Recent developments.

Integrated microfluidic devices

The detection and recognition of objects in images is a key research topic in the computer vision community Within this area, face recognition and interpretation has attracted increasing attention owing to the possibility of unveiling human perception mechanisms, and for the development of practical biometric systems This book and the accompanying website, focus on template matching, a subset of object recognition techniques of wide applicability, which has proved to be particularly effective for face recognition applications Using examples from face processing tasks throughout the book to illustrate more general object recognition approaches, Roberto Brunelli: examines the basics of digital image formation, highlighting points critical to the task of template matching; presents basic and advanced template matching techniques, targeting grey-level images, shapes and point sets; discusses recent pattern classification paradigms from a template matching perspective; illustrates the development of a real face recognition system; explores the use of advanced computer graphics techniques in the development of computer vision algorithms Template Matching Techniques in Computer Vision is primarily aimed at practitioners working on the development of systems for effective object recognition such as biometrics, robot navigation, multimedia retrieval and landmark detection It is also of interest to graduate students undertaking studies in these areas

Template Matching Techniques in Computer Vision: Theory and Practice

We report on our activities in design, fabrication, characterization and system integration of refractive microlens arrays for sensors and microsystems. Examples for chemical analysis systems (, blood gas sensor), neural networks and multiple pupil imaging systems for photolithography (microlens and smart mask lithography) are presented.

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Design, fabrication and testing of microlens arrays for sensors and microsystems

A new optical concept for compact digital image acquisition devices with large field of view is developed and proofed experimentally. Archetypes for the imaging system are compound eyes of small insects and the Gabor-Superlens. A paraxial 3x3 matrix formalism is used to describe the telescope arrangement of three microlens arrays with different pitch to find first order parameters of the imaging system. A 2mm thin imaging system with 21x3 channels, 70 masculinex10 masculine field of view and 4.5mm x 0.5mm image size is optimized and analyzed using sequential and non-sequential raytracing and fabricated by microoptics technology. Anamorphic lenses, where the parameters are a function of the considered optical channel, are used to achieve a homogeneous optical performance over the whole field of view. Captured images are presented and compared to simulation results.

Microoptical telescope compound eye

We review the most important fabrication techniques for glass and plastic refractive microlenses and we quantitatively characterize in a systematic way the corresponding state-of-the-art microlenses, which we obtained from selected research groups. For all our measurements we rely on three optical instruments: a non-contact optical profiler, a transmission Mach–Zehnder interferometer and a Twyman–Green interferometer. To conclude, we survey and discuss the different fabrication techniques by comparing the geometrical and optical characteristics of the microlenses, the range of materials in which the lenses can be produced, their potential for low-cost fabrication through mass-replication techniques and their suitability for monolithic integration with other micro-optical components.

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Comparing glass and plastic refractive microlenses fabricated with different technologies

Miniaturized imaging systems

A new concept for a flat digital image acquisition device for large field of views (FOV) has been developed. Antetypes for the optical system are compound eyes of small insects and the Gabor-Superlens. A paraxial 3x3 matrix formalism is used to describe the arrangement of three microlens arrays (MLA) with different pitches to find the first order parameters of the system. These considerations are extended to arrays of anamorphic lenses with variable parameters to achieve homogeneous optical performance over the whole FOV. The model is validated by implementation of different systems into commercial raytracing software. A trade-off between system length, sensitivity and diffraction limited resolution as well as aberrations is discussed.

Reinhard Völkel

Papers

Design, fabrication and testing of microlens arrays for sensors and microsystems

Microoptical telescope compound eye

Comparing glass and plastic refractive microlenses fabricated with different technologies

Miniaturized imaging systems

Theoretical analysis of an artificial superposition compound eye for application in ultra flat digital image acquisition devices