R
Richard Schlegel
Researcher at Georgetown University Medical Center
Publications - 191
Citations - 15599
Richard Schlegel is an academic researcher from Georgetown University Medical Center. The author has contributed to research in topics: Cell culture & Gene. The author has an hindex of 65, co-authored 183 publications receiving 14636 citations. Previous affiliations of Richard Schlegel include Tufts Medical Center & Laboratory of Molecular Biology.
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The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes.
TL;DR: Both the full-length E6 and E7 genes were required for the induction of keratinocyte immortalization and resistance to terminal differentiation and mutation of either gene in the context of this recombinant plasmid eliminated the ability to induce stable differentiation-resistant transformants.
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Systemic immunization with papillomavirus L1 protein completely prevents the development of viral mucosal papillomas
JoAnn Suzich,Shin-je Ghim,Frances J. Palmer-Hill,Wendy I. White,J. K. Tamura,J. A. Bell,J. A. Newsome,A. B. Jenson,Richard Schlegel +8 more
TL;DR: The results indicate the feasibility of developing a human vaccine to prevent mucosal papillomas, which can progress to malignancy, and suggest adjuvants appeared useful for prolonging the host immune response.
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ROCK Inhibitor and Feeder Cells Induce the Conditional Reprogramming of Epithelial Cells
Xuefeng Liu,Virginie Ory,Sandra Chapman,Hang Yuan,Chris Albanese,Bhaskar Kallakury,Olga A. Timofeeva,Caitlin Nealon,Aleksandra Dakic,Vera Simic,Bassem R. Haddad,Johng S. Rhim,Anatoly Dritschilo,Anna T. Riegel,Alison A. McBride,Richard Schlegel +15 more
TL;DR: It is demonstrated that a Rho kinase inhibitor (Y-27632), in combination with fibroblast feeder cells, induces normal and tumor epithelial cells from many tissues to proliferate indefinitely in vitro, without transduction of exogenous viral or cellular genes.
Journal Article
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
TL;DR: Six cell lines established from human squamous-cell carcinomas of the bladder, pharynx, lung, esophagus, and vulva were negative for HPV-6, HPV-11, HPV -16, and HPV-18 DNA sequences under stringent hybridization conditions.
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TGF-β1 inhibition of c-myc transcription and growth in keratinocytes is abrogated by viral transforming proteins with pRB binding domains
Jennifer A. Pietenpol,Roland Stein,Elizabeth Moran,Peter Yaciuk,Richard Schlegel,Russette M. Lyons,Mark R. Pittelkow,Karl Münger,Peter M. Howley,Harold L. Moses +9 more
TL;DR: Observations indicate that pRB or another protein that interacts with this binding domain mediates TGF-beta 1 regulation of c-myc gene expression and growth inhibition.